Naslov
Method for the determination of percutaneous absorption of polyethylene glycols of different molecular weights in volunteers by tape stripping

Autori
Jakasa, Ivone ; Calkoen, Florentine ; Kezic, Sanja

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Skup
Stratum Corneum IV

Mjesto i datum
Pariz, Francuska, 17.06.2004. - 19.06.2004

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
percutaneous penetration; polyethylene glycols; stratum corneum

Sažetak
Objectives: The aim of the project is to develop a method for determination of percutaneous penetration of polyethylene glycols (PEGs) of different molecular weight (MW) through the stratum corneum (SC). The method will be used to study the differences in percutaneous penetration between normal and impaired skin (e.g. by patients with atopic dermatitis or by skin impaired with sodium lauryl sulfate). Methods: PEGs are extensively used in research of intestinal permeability. The water/octanol partitioning (Po/w) coefficient doesn’ t change greatly with molecular mass which justifies their use as appropriate molecular size model compound not compounded by lipophilicity. This is for the first time that percutaneous penetration of PEGs has been determined in human skin in vivo. The water mixture of PEG containing different oligomers of MW of 150-700 Da was applied onto the lower forearm using round Finn Patch test chambers with a diameter of 18 mm for 6 and 24 hours. 15 minutes after the end of exposure, pieces of adhesive tape, were consecutively applied to the test sites. Tapes were consecutively applied until the SC is totally removed as observed by the shinny appearance of the skin and by measuring the trans-epidermal water loss. The concentration of PEG’ s in tape strips was done using gas chromatography. To normalize for the amount of the SC and to assess the depth of the consecutive SC strip, the proteins were analyzed in each tape strip. The results were expressed as amount of each oligomer /amount of proteins vs. SC depth. The function representing the Fick’ s second law of diffusion was fitted to the data enabling the calculation of diffusion parameters. Results: The developed method allowed us to measure the concentration of PEGs with MW up to 650 Da. Preliminary results showed that the MW cut off is shifted to higher MW in the skin pre-treated with SLS and that the amount of PEGs penetrated the SC was significantly higher than in non-treated skin.

Izvorni jezik
Engleski

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