Naslov
Non-invasive prenatal paternity testing from maternal blood

Autori
Wagner, Jasenka ; Džijan, Snježana ; Marjanović, Damir ; Lauc, Gordan

Izvornik
International Journal of Legal Medicine (0937-9827) 123 (2009), 1; 75-79

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
prenatal paternity testing; cell-free fetal DNA; STR loci

Sažetak
Prenatal paternity analysis can be performed only after invasive sampling of chorionic villi or amnionic fluid. Aiming to enable noninvasive paternity testing we attempted to amplify fetal alleles from maternal plasma. Cell-free DNA was isolated from plasma of 20 pregnant women and amplified with ampFLSTR Identifiler and ampFLSTR Yfiler kits. Unfortunately autosomal fetal alleles were heavily suppressed by maternal DNA and the only locus that was reliably amplified with AmpFLSTR Identifiler kit was amelogenin, which revealed only fetal gender. Much better success was obtained with AmpFLSTR Yfiler kit which, in the case of male fetuses, successfully amplified between 6 and 16 fetal loci. All amplified fetal alleles matched the alleles of their putative fathers, confirming the tested paternity. To the best of our knowledge this is a first report of noninvasive prenatal paternity testing.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti

POVEZANOST RADA