Pregled bibliografske jedinice broj: 37622
Protein-bound DNA ends in Streptomyces rimosus
Protein-bound DNA ends in Streptomyces rimosus // Abstracts of the 11th International Symposium on the Biology of Actinomycetes
Sisi : Heraklion: Hellenic Society of Biological Sciences, 1999. str. 98-98 (poster, međunarodna recenzija, sažetak, znanstveni)
CROSBI ID: 37622 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Protein-bound DNA ends in Streptomyces rimosus
Autori
Horvat, Lada-Ivana ; Stoll, Alexander ; Cullum, John ; Hranueli, Daslav
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
Abstracts of the 11th International Symposium on the Biology of Actinomycetes
/ - Sisi : Heraklion : Hellenic Society of Biological Sciences, 1999, 98-98
Skup
11th International Symposium on the Biology of Actinomycetes
Mjesto i datum
Mália, Grčka; Heraklion, Grčka, 24.10.1999. - 28.10.1999
Vrsta sudjelovanja
Poster
Vrsta recenzije
Međunarodna recenzija
Ključne riječi
Streptomyces rimosus; linear plasmid; end fragments; protein-bound linear ends
Sažetak
The early strain R6-65 and a later derivative R6-501 from the oxytetracycline development programme of the "Zagreb" strain of S. rimosus have been compared. The linear chromosomal restriction map of R6-501 was established in earlier work and was 8 Mb in size with terminal inverted repeats (TIR) of 550 kb. The digestion pattern of the R6-65 chromosome was indistinguishable. Previous restriction mapping with several enzymes showed that the 310 kb linear plasmid in R6-65 (pPZG102) is smaller than the 387 kb plasmid in R6-501 (pPZG101). No TIRs could be detected in pPZG102 by restriction mapping. pPZG101 appears to have been derived from pPZG102 by a recombination event that generated terminal inverted repeats of 180 kb. Restriction fragments corresponding to protein-bound linear ends were isolated and visualised in agarose gels. As had been expected from the presence of the chromosome with a TIR and pPZG102 without a TIR, R6-65 gave rise to three end fragments. Surprisingly, R6-501, in which both the plasmid and chromosome have a TIR, also gave three end fragments. From the restriction mapping, it had been expected that the end fragments in R6-501 would be identical in size to those in R6-65. However, all three of the fragments differed in size. Work is underway to assign the ends to molecules in the cell mapped by pulsed-field gel electrophoresis and to determine the nature of the changes between R6-65 and its descendant R6-501 using DNA sequencing.
Izvorni jezik
Engleski
Znanstvena područja
Prehrambena tehnologija
