Naslov
Inaktivacija CYP38 proteina uzrokuje promjene u fotosintetskom prijelazu stanja
(CYP38 mutant plants display impaired state 2 transitions)

Autori
Tomašić, Ana ; Jurić, Snježana ; Lepeduš, Hrvoje ; Prebeg, Tatjana ; Allen, John F. ; Fulgosi, Hrvoje

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
50 godina molekularne biologije u Hrvatskoj - Zbornik sažetaka / Zahradka, Ksenija ; Plohl, Miroslav ; Ambriović-Ristov, Andreja (ur.).- Zagreb : Institut Ruđer Bošković , 2008.72-72 (ISBN:978-953-6690-78-7) / - , 2008

Skup
50 godina molekularne biologije u Hrvatskoj

Mjesto i datum
Zagreb, Hrvatska, 20.11.2008. - 21.11.2008

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
Arabidopsis; fotosinteza; PSII; fosforilacija; fotosintetski prijelaz stanja
(Arabidopsis; photosynthesis; PSII; phosphorylation; state-transitions)

Sažetak
Chloroplasts, semiautonomous organelles in plant cells, are responsible for the process of photosynthesis. Chloroplast protein TLP40 (thylakoid lumen PPIase of 40 kDa), cychlophilin-like PPIase, located in thylakoid lumen shows peptidyl-prolyl cis-trans isomerase protein folding activity characteristic of immunophilins and was co-isolated with thylakoid membrane phosphatase from spinach. Gene coding CYP38 protein, ortholog of TLP40 from spinach, is located on the 3rd chromosome of Arabidopsis thaliana. To characterize the function of this protein in vivo, we analyzed knock-out Arabidopsis thaliana lines: cyp38-1 and cyp38-2, which were screened for T-DNA insertion. Gene was interrupted by T-DNA insertion at position 1962 (6th exon) in cyp38-2, as confirmed by PCR and tested by Western blotting. Chlorophyll fluorescence measured in vivo with MINI-PAM portable chlorophyll fluorometer (Waltz) indicated slightly elevated non-photochemical quenching (NPQ) and considerably lowered relative electron transport rate (ETR) of mutants compared to wild-type plants. Also, PSII efficiency measured (Fv/Fm ratio), under normal light conditions was decreased in mutants. A quantitative state-transition measurements of intact rosetta leaves revealed that CYP38 gene inactivation leads to impaired state 2 transition implicating possible defects of antenna dephosphorylation in mutants lacking CYP38 protein.

Izvorni jezik
Hrvatski

Znanstvena područja
Biologija

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