Naslov
LXR-agonists regulate ApoM expression differentially in liver and intestine

Autori
Calayir, Ermine ; Becker, Tatjana M. ; Kratzer, Adelheid ; Ebner, Brigit ; Panzenboeck, Ute ; Štefulj, Jasminka ; Kostner, Gerhard M.

Izvornik
Current pharmaceutical biotechnology (1389-2010) 9 (2008), 6; 516-521

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
intestine ; lipid metabolism ; lipocalin ; expression profiling

Sažetak
Apolipoprotein M (apoM) has been suggested to play a role in reverse cholesterol transport. Here we studied the influence of liver X-receptor (LXR) agonist on the transcriptional regulation of apoM. Studies were performed in murine liver and intestinal mucosal cells in vivo and in human intestinal Caco-2 cells in vitro. The expression of apoM was analyzed by quantitative real time PCR, and compared to well-established LXR target genes. Mice fed with TO901317 for six days showed a downregulation of apoM and apoAI in the liver to 40 % and 60 % respectively and an upregulation of Cyp7A1 to 280 %. In the small intestine, however, apoM and apoAI were upregulated by 30-60 % and ABCA1 by 250-430 %. In Caco-2 cells TO901317 caused a 60 % upregulation and the natural LXR agonist 22-hydroxycholesterol a 40 % upregulation of apoM. Possible causes for the differential effects in liver and intestine are discussed.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti

POVEZANOST RADA