Pregled bibliografske jedinice broj: 375028
RNA polymerase II-associated proteins are required for a DNA conformation change in the transcription initiation complex
RNA polymerase II-associated proteins are required for a DNA conformation change in the transcription initiation complex // Proceedings of the National Academy of Sciences of the United States of America, 88 (1991), 17; 7509-7513 (podatak o recenziji nije dostupan, članak, znanstveni)
CROSBI ID: 375028 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
RNA polymerase II-associated proteins are required for a DNA conformation change in the transcription initiation complex
Autori
Buratowski, S. ; Sopta, Mary ; Greenblatt, J. ; Sharp, P.A.
Izvornik
Proceedings of the National Academy of Sciences of the United States of America (0027-8424) 88
(1991), 17;
7509-7513
Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni
Ključne riječi
transcription initiation; RNA polymerase II
Sažetak
Proteins purified on the basis of their affinity for RNA polymerase II effectively substitute for previously defined transcription initiation factors. In two assays, formation of initiation complexes and transcription in vitro, the RNA polymerase II-associated proteins behaved identically to a fraction containing transcription factors IIE and IIF. Both fractions greatly stabilized the association of polymerase with the promoter and were required for the formation of complete initiation complexes. By using the DNA-cleaving reagent phenanthroline.copper in footprinting reactions, the RNA polymerase II-associated proteins were shown to be required for a DNA conformation change near the initiation site of the promoter. Based on similarity to the prokaryotic transcription complex, this conformation change is likely to represent a transition from a closed to an open complex.
Izvorni jezik
Engleski
