Naslov
Nm23-SD1 protein from marine sponge Suberites domuncula

Autori
Perina, Dragutin ; Herak Bosnar, Maja ; Mikoč, Andreja ; Bago, Ružica ; Müller, Werner E.G. ; Ćetković, Helena

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
HDBMB2008 Congress of the Croatian Society of Biochemistry and Molecular Biology with international participation / Strelec, Ivica ; Glavaš-Obrovac, Ljubica - Osijek : Hrvatsko Društvo za Biotehnologiju, 2008

ISBN
978-953-95551-2-0

Skup
Congress of the Croatian Society of Biochemistry and Molecular Biology 2008

Mjesto i datum
Osijek, Hrvatska, 17.10.2008. - 20.10.2008

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
sponge; nm23

Sažetak
Nucleoside diphosphate kinases Nm23/NDPK are enzymes that are conserved through evolution and are present in all three domains of life: Bacteria, Archaea and Eukarya. The human nm23-H1/NDPK was recognized as a first metastasis suppressor gene and is the most studied member of Nm23/NDPK family of genes/proteins. Suberites domuncula is a member of the most ancient and simplest extant phylum of multicellular animals – Sponges (Porifera). Database of S. domucula expressed sequence tags (ESTs) was recently constructed by random cDNA sequencing. Herein we report new evidence on sponge Suberites domuncula NDPK/Nm23. The sponge protein was named Nm23-SD1, because it displays the highest sequence similarity with human Nm23-H1 protein. The overall sequence homology of Nm23-SD1 and human Nm23-H1 is very high - 79% (71% identity). The secondary structure prediction for sponge and human Nm23 protein pair is almost identical. The goal of this study was to reveal the characteristics of the sponge gene/protein and discover whether the sponge Nm23-SD1 can replace the antimetastatic function of the human homolog in human metastatic breast cancer cell line MDA-MB-231T. Sponge nm23-SD1 gene has three introns of which the first two are on the same positions as the human introns. Nm23-SD1 gene probably reflects the archetype of the metazoan NDP kinase gene. Nm23-SD1 cDNA was cloned and overexpressed using pET15b and E. coli as a host. Purified protein showed nonspecific DNA-binding activity and had a similar level of kinase activity as its human homolog. Most Nm23-SD1 oligomers are in the hexamer form, same as the human Nm23-H1. The preliminary results reveal that the sponge Nm23-SD1 might have a similar cellular localization as the human Nm23-H1. Stable clones expressing sponge Nm23-SD1 are currently under construction.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

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