ࡱ> /1.5@<bjbj22 .XX< 2222222F  F$RZJ222???22??"?a22a EP"a0a  aFF2222a 2u(Z @?L4}FF$j$5 FFjSu-P062 IMMUNOASSAYING OF BONE MORPHOGENETIC PROTEIN-9 AND ITS RECEPTORS IN LIVER AND PANCREAS OF DIABETIC WISTAR RATS O. Cvijanovic*1, T. Celic1, S. Peternel2, I. Maric1, Z. Crncevic - Orlic3, T. Turk3, D. Bobinac1 1Department of Anatomy, 2Department of Pharmacology, Medical Faculty University in Rijeka, 3Department of Internal Medicine, Clinical Hospital Rijeka, Rijeka, Croatia IMMUNOASSAYING OF BONE MORPHOGENETIC PROTEIN-9 AND ITS RECEPTORS IN LIVER AND PANCREAS OF DIABETIC WISTAR RATS Background data: In type 2 diabetes, elevated glucose concentration results from impaired secretion of insulin and insulin resistance in target tissues like muscle and liver. It is possible that liver generates hepatic insulin-sensitizing substance (HISS) which enhances glucose uptake in peripheral tissues, but the nature of this putative hormone remains obscure. BMP-9 molecule identified by Chen et al1 is very strong candidate for HISS, with an observed effect that was comparable to that seen with insulin. Moreover, purified recombinant BMP-9 was shown to cause a sustained lowering of plasma glucose concentrations in normal (C57BL/6) and diabetic (db/db) mice. In addition to mimicking the action of insulin, BMP-9 also stimulated insulin release in Wistar and Zucker diabetic rats. Despite to encouraging results of BMP-9 as potential HISS, questions remain regarding physiological role of BMP-9 in glucose metabolism. Aim: To explore immunolocalisation of BMP-9 and BMP receptors (BMPR-IA, BMPR-IB and BMPR-II) in liver and pancreas of diabetic Wistar rat (N=8) and also in control group (N=8). Methods: Diabetes was induced by single streptozotocin administration.Glucose concentrations were controlled in regular bases and animals were sacrificed by the end of the fifth week. Immunolocalisation was performed using anti-BMP-9, anti BMPR-IA, anti-BMPR-IB and anti BMPR-II goat polyclonal antibody (Santa Cruz Biotechnology, CA, USA). Results: BMP-9, BMPR-IA, BMPR-IB and BMPR-II are expressed in both normal and diabetic organs. BMP-9 activity as well as activity of BMPR-IA, BMPR-IB and BMPR-II was observed as cytoplasmic staining in hepatocytes, epithelial cells of bilial ducts and in Kupfer cells. In pancreas, activity of BMP-9, BMPR-IA, BMPR-IB and BMPR-II was detected in epithelial cells of pancreatic ducts and also in epithelial cells of Langerhans islets. Conclusions: BMP-9 and BMP receptors are expressed in the diabetic rat liver and pancreas. Further determination of the expression levels of the BMP-9 and it's receptors in organs mostly affected by chronic hyperglycemia will better explain this protein's possible protective function in diabetes. 1. Chen C et al. An integrated functional genomics screening program reveals a role for BMP-9 in glucose homeostasis. Nat Biotechnol. 2003 Mar;21(3):294-301. Conflict of Interest: None declared xy5 6 +:;<轮hYy*hD5B*phhYy*6B*OJQJ]ph hYy*6B*H*OJQJ]phhYy*B*H*OJQJphhYy*B*OJQJphhYy*B*phhYy*5B*OJQJ\ph<<,1h. A!"#$% @@@ NormalCJ_HaJmH sH tHDA@D Default Paragraph FontRi@R  Table Normal4 l4a (k@(No List< > 0> < < <   4 T6 6 7  ,3tt> ~~> 9*urn:schemas-microsoft-com:office:smarttagsStateh*urn:schemas-microsoft-com:office:smarttagsCity0http://www.5iamas-microsoft-com:office:smarttagsV*urn:schemas-microsoft-com:office:smarttagsplacehttp://www.5iantlavalamp.com/B*urn:schemas-microsoft-com:office:smarttagscountry-region   > 33  > >  Biblioteka_40zLD5Yy*NK|@    @{< @UnknownGz Times New Roman5Symbol3& z Arial3Times"1JˆKˆ  !247 7 3H)?K|xSu-P062 IMMUNOASSAYING OF BONE MORPHOGENETIC PROTEIN-9 AND ITS RECEPTORS IN LIVER AND PANCREAS OF DIABETIC WISTAR RATS Biblioteka_4 Biblioteka_4Oh+'0(@LXl    ySu-P062 IMMUNOASSAYING OF BONE MORPHOGENETIC PROTEIN-9 AND ITS RECEPTORS IN LIVER AND PANCREAS OF DIABETIC WISTAR RATSRu-P Biblioteka_4UNOiblibl Normal.dot4 Biblioteka_4UNO1blMicrosoft Word 10.0@F#@P@5P ՜.+,0` hp|  A67 A ySu-P062 IMMUNOASSAYING OF BONE MORPHOGENETIC PROTEIN-9 AND ITS RECEPTORS IN LIVER AND PANCREAS OF DIABETIC WISTAR RATS Title  !"#$%'()*+,-0Root Entry F`#EP2Data 1TableWordDocument.SummaryInformation(DocumentSummaryInformation8&CompObjj  FMicrosoft Word Document MSWordDocWord.Document.89q