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Pregled bibliografske jedinice broj: 373629

RecJ nuclease is required for SOS induction after introduction of a double-strand break in a RecA loading deficient recB mutant of Escherichia coli

Vlašić, Ignacija; Ivančić-Baće, Ivana; Imešek, Mirna; Mihaljević, Boris; Brčić-Kostić, Krunoslav
RecJ nuclease is required for SOS induction after introduction of a double-strand break in a RecA loading deficient recB mutant of Escherichia coli // Biochimie, 90 (2008), 9; 1347-1355 doi:10.1016/j.biochi.2008.04.002 (međunarodna recenzija, članak, znanstveni)

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Naslov
RecJ nuclease is required for SOS induction after introduction of a double-strand break in a RecA loading deficient recB mutant of Escherichia coli

Autori
Vlašić, Ignacija ; Ivančić-Baće, Ivana ; Imešek, Mirna ; Mihaljević, Boris ; Brčić-Kostić, Krunoslav

Izvornik
Biochimie (0300-9084) 90 (2008), 9; 1347-1355

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
RecJ; SOS response; RecBCD; E. coli

Sažetak
The SOS response is an important mechanism which allows Escherichia coli cells to maintain genome integrity. Two key proteins in SOS regulation are LexA (repressor) and RecA (coprotease). The signal for SOS induction is generated at the level of a RecA filament. Depending on the type of DNA damage, a RecA filament is produced by specific activities (helicase, nuclease and RecA loading) of either RecBCD, RecF or a hybrid recombination pathway. It was recently demonstrated that RecA loading activity is essential for the induction of the SOS response after UV-irradiation. In this paper we studied the genetic requirements for SOS induction after introduction of a double-strand break (DSB) by the I-SceI endonuclease in a RecA loading deficient recB mutant (recB1080). We monitored SOS induction by assaying beta-galactosidase activity and compared induction of the response between strains having one or more inactivated mechanisms of RecA loading and their derivatives. We found that simultaneous inactivation of both RecA loading functions (in recB1080 recO double mutant) partially impairs SOS induction after introduction of a DSB. However, we found that the RecJ nuclease is essential for SOS induction after the introduction of a DSB in the recB1080 mutant. This result indicates that RecJ is needed to prepare ssDNA for subsequent loading of RecA protein. It implies that an additional type of RecA loading could exist in the cell.

Izvorni jezik
Engleski

Znanstvena područja
Biologija



POVEZANOST RADA

Projekti:
098-0982913-2867 - Uloga rekombinacije u popravku DNA i evoluciji genoma (Brčić-Kostić, Krunoslav, MZOS ) ( CroRIS)
119-1191196-1201 - Organizacija, funkcija i mehanizmi evolucije biljnog genoma (Besendorfer, Višnja, MZOS ) ( CroRIS)

Ustanove:
Institut "Ruđer Bošković", Zagreb,
Prirodoslovno-matematički fakultet, Zagreb

Profili:

Avatar Url Boris Mihaljević (autor)

Avatar Url Mirna Halasz (autor)

Avatar Url Krunoslav Brčić-Kostić (autor)

Avatar Url Ignacija Vlašić (autor)

Avatar Url Ivana Ivančić Baće (autor)

Poveznice na cjeloviti tekst rada:

Pristup cjelovitom tekstu rada doi www.sciencedirect.com dx.doi.org

Citiraj ovu publikaciju:

Vlašić, Ignacija; Ivančić-Baće, Ivana; Imešek, Mirna; Mihaljević, Boris; Brčić-Kostić, Krunoslav
RecJ nuclease is required for SOS induction after introduction of a double-strand break in a RecA loading deficient recB mutant of Escherichia coli // Biochimie, 90 (2008), 9; 1347-1355 doi:10.1016/j.biochi.2008.04.002 (međunarodna recenzija, članak, znanstveni)
Vlašić, I., Ivančić-Baće, I., Imešek, M., Mihaljević, B. & Brčić-Kostić, K. (2008) RecJ nuclease is required for SOS induction after introduction of a double-strand break in a RecA loading deficient recB mutant of Escherichia coli. Biochimie, 90 (9), 1347-1355 doi:10.1016/j.biochi.2008.04.002.
@article{article, author = {Vla\v{s}i\'{c}, Ignacija and Ivan\v{c}i\'{c}-Ba\'{c}e, Ivana and Ime\v{s}ek, Mirna and Mihaljevi\'{c}, Boris and Br\v{c}i\'{c}-Kosti\'{c}, Krunoslav}, year = {2008}, pages = {1347-1355}, DOI = {10.1016/j.biochi.2008.04.002}, keywords = {RecJ, SOS response, RecBCD, E. coli}, journal = {Biochimie}, doi = {10.1016/j.biochi.2008.04.002}, volume = {90}, number = {9}, issn = {0300-9084}, title = {RecJ nuclease is required for SOS induction after introduction of a double-strand break in a RecA loading deficient recB mutant of Escherichia coli}, keyword = {RecJ, SOS response, RecBCD, E. coli} }
@article{article, author = {Vla\v{s}i\'{c}, Ignacija and Ivan\v{c}i\'{c}-Ba\'{c}e, Ivana and Ime\v{s}ek, Mirna and Mihaljevi\'{c}, Boris and Br\v{c}i\'{c}-Kosti\'{c}, Krunoslav}, year = {2008}, pages = {1347-1355}, DOI = {10.1016/j.biochi.2008.04.002}, keywords = {RecJ, SOS response, RecBCD, E. coli}, journal = {Biochimie}, doi = {10.1016/j.biochi.2008.04.002}, volume = {90}, number = {9}, issn = {0300-9084}, title = {RecJ nuclease is required for SOS induction after introduction of a double-strand break in a RecA loading deficient recB mutant of Escherichia coli}, keyword = {RecJ, SOS response, RecBCD, E. coli} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE

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