Naslov
Long range organization of satellite DNAs in the genome of Tribolium madens (Coleoptera, Insecta)

Autori
Durajlija-Žinić, Sonja

Vrsta, podvrsta i kategorija rada
Ocjenski radovi, doktorska disertacija

Fakultet
Prirodoslovno-matematički fakultet

Mjesto
Zagreb

Datum
15.07

Godina
1999

Stranica
114

Mentor
Ugarković, Đurđica

Ključne riječi
extended chromosomes; extended DNA fibers; long range organization; pulsed field gel electrophoresis; satellite DNA; two-color florescence in situ hybridization

Sažetak
Satellite DNAs are tandemly arranged highly repetitive DNA sequences that are found in genomes of all eukaryotes. Long range organization of these sequences is poorly understood. The work presented here represents the first insight into the large scale organization of satellite DNAs in insect family Tenebrionidae. Species Tribolium madens was chosen for this study because of the presence of two related satellites, and because of the existence of supernumerary chromosomes. In order to study organization of satellites I and II on long genomic segments, the method of pulsed field gel electrophoresis was performed together with fluorescence in situ hybridization on metaphase chromosomes, extended chromosomes and on extended DNA fibers. Results reveal the presence of both satellites in pericentromeric heterochromatin of all chromosomes including supernumeraries. It has been shown that two satellites are closely adjacent and that they form long uninterrupted clusters that range in size from 100 kb up to several Mb. It has been found that satellite II exhibits long range organization unusual for satellite DNA.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA