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Pregled bibliografske jedinice broj: 372135

Cytogenetic status and oxidative DNA-damage induced by atorvastatin in human peripheral blood lymphocytes: standard and Fpg-modified comet assay

Gajski, Goran; Garaj-Vrhovac, Vera; Oreščanin, Višnja
Cytogenetic status and oxidative DNA-damage induced by atorvastatin in human peripheral blood lymphocytes: standard and Fpg-modified comet assay // Toxicology and Applied Pharmacology, 231 (2008), 1; 85-93 doi:10.1016/j.taap. (međunarodna recenzija, članak, znanstveni)

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Naslov
Cytogenetic status and oxidative DNA-damage induced by atorvastatin in human peripheral blood lymphocytes: standard and Fpg-modified comet assay

Autori
Gajski, Goran ; Garaj-Vrhovac, Vera ; Oreščanin, Višnja

Izvornik
Toxicology and Applied Pharmacology (0041-008X) 231 (2008), 1; 85-93

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
atorvastatin; oxidative DNA-damage; comet assay; Fpg-modified comet assay; micronucleus test; peripheral blood human lymphocytes

Sažetak
To investigate the genotoxic potential of atorvastatin on human lymphocytes in vitro standard comet assay was used in the evaluation of basal DNA damage and to investigate possible oxidative DNA damage produced by reactive oxygen species (ROS) Fpg-modified version of comet assay was also conducted. In addition to these techniques the new criteria for scoring micronucleus test were applied for more complete detection of baseline damage in binuclear lymphocytes exposed to atorvastatin 80 mg/day in different time periods by virtue of measuring the frequency of micronuclei, nucleoplasmic bridges and nuclear buds. All parameters obtained with the standard comet assay and Fpg-modified comet assay were significantly higher in the treated than in control lymphocytes. The Fpg-modified comet assay showed a significantly greater tail length, tail intensity, and tail moment in all treated lymphocytes than did the standard comet assay, which suggests that oxidative stress is likely to be responsible for DNA damage. DNA damage detected by the standard comet assay indicates that some other mechanism is also involved. In addition to the comet assay, a total number of micronuclei, nucleoplasmic bridges and nuclear buds were significantly higher in the exposed than in controlled lymphocytes. Regression analyses showed a positive correlation between the results obtained by the comet (Fpg-modified and standard) and micronucleus assay. Overall, the study demonstrated that atorvastatin in its highest dose is capable of producing damage on the level of DNA molecule and cell.

Izvorni jezik
Engleski

Znanstvena područja
Biologija



POVEZANOST RADA

Projekti:
022-0222148-2125 - Mutageni i antimutageni u ekogenetičkim istraživanjima (Garaj-Vrhovac, Vera, MZOS ) ( CroRIS)

Ustanove:
Institut za medicinska istraživanja i medicinu rada, Zagreb

Profili:

Avatar Url Vera Garaj-Vrhovac (autor)

Avatar Url Goran Gajski (autor)

Poveznice na cjeloviti tekst rada:

doi

Citiraj ovu publikaciju:

Gajski, Goran; Garaj-Vrhovac, Vera; Oreščanin, Višnja
Cytogenetic status and oxidative DNA-damage induced by atorvastatin in human peripheral blood lymphocytes: standard and Fpg-modified comet assay // Toxicology and Applied Pharmacology, 231 (2008), 1; 85-93 doi:10.1016/j.taap. (međunarodna recenzija, članak, znanstveni)
Gajski, G., Garaj-Vrhovac, V. & Oreščanin, V. (2008) Cytogenetic status and oxidative DNA-damage induced by atorvastatin in human peripheral blood lymphocytes: standard and Fpg-modified comet assay. Toxicology and Applied Pharmacology, 231 (1), 85-93 doi:10.1016/j.taap..
@article{article, author = {Gajski, Goran and Garaj-Vrhovac, Vera and Ore\v{s}\v{c}anin, Vi\v{s}nja}, year = {2008}, pages = {85-93}, DOI = {10.1016/j.taap.}, keywords = {atorvastatin, oxidative DNA-damage, comet assay, Fpg-modified comet assay, micronucleus test, peripheral blood human lymphocytes}, journal = {Toxicology and Applied Pharmacology}, doi = {10.1016/j.taap.}, volume = {231}, number = {1}, issn = {0041-008X}, title = {Cytogenetic status and oxidative DNA-damage induced by atorvastatin in human peripheral blood lymphocytes: standard and Fpg-modified comet assay}, keyword = {atorvastatin, oxidative DNA-damage, comet assay, Fpg-modified comet assay, micronucleus test, peripheral blood human lymphocytes} }
@article{article, author = {Gajski, Goran and Garaj-Vrhovac, Vera and Ore\v{s}\v{c}anin, Vi\v{s}nja}, year = {2008}, pages = {85-93}, DOI = {10.1016/j.taap.}, keywords = {atorvastatin, oxidative DNA-damage, comet assay, Fpg-modified comet assay, micronucleus test, peripheral blood human lymphocytes}, journal = {Toxicology and Applied Pharmacology}, doi = {10.1016/j.taap.}, volume = {231}, number = {1}, issn = {0041-008X}, title = {Cytogenetic status and oxidative DNA-damage induced by atorvastatin in human peripheral blood lymphocytes: standard and Fpg-modified comet assay}, keyword = {atorvastatin, oxidative DNA-damage, comet assay, Fpg-modified comet assay, micronucleus test, peripheral blood human lymphocytes} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE

Uključenost u ostale bibliografske baze podataka::


  • Biological Sciences

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