Naslov
Hepatic Phospholipid Fatty Acids Profile in Mice Infected with Listeria monocytogenes

Autori
Marušić, Sanela ; Bačić, Gordana ; Bubonja, Marina ; Giacometti, Jasminka ; Abram, Maja

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Skup
4th Croatian Congress of Microbiology with International Participation

Mjesto i datum
Zadar, Hrvatska, 24.09.2008. - 27.09.2008

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
Listeria monocytogenes; hepatic phospholipid fatty acids; mice

Sažetak
Listeria monocytogenes is ubiquitous, gram-positive bacteria that causes sepsis and meningitis in immunocompromised patients and a serious maternal/fetal infection in pregnant women. With a mortality rate of 20-30%, listeriosis is the leading cause of death from all food-borne pathogens. Murine listeriosis has proven to be an useful experimental model for defining mechanisms that underlie immunity and host defenses to infection. When administered intravenously, L. monocytogenes will initially be found in many organs but concentrates into the liver, with hepatocytes being the principal site of bacterial multiplication. The aim of this study was to show changes in phospholipids (PL) and their fatty acids (FA) composition in the liver of Balb/c mice upon infection by L. monocytogenes. The kinetics of bacterial clearance, histopathological changes, as well as dynamics of the phospholipid fatty acids (PLFAs) in the liver tissue were followed during one week post infection. All infected animals exhibited hepatitis according to histopathological changes and elevated aminotransferases levels. Also, differences in the liver PLFAs composition in infected versus control mice were also found. Significant increase in the n-3/n-6 ratio led to diminish proinflammatory eicosanoid biosynthesis as well as T cell proliferation. Diminished inflammatory responses in listeriosis may affect host infectious disease resistance. Therefore, phospholipids and fatty acids composition can serve as estimates of structural-functional description in the host-microbe relationship and should be further examined.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Kliničke medicinske znanosti

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