Pregled bibliografske jedinice broj: 371870
Molecular-cytogenetical characterization of the natural hybrids and polyploids of common onion Alluim cepa L.
Molecular-cytogenetical characterization of the natural hybrids and polyploids of common onion Alluim cepa L. // Proceedings of Abstacts of the Sixth Congress of Croatian Biologists
Opatija, Hrvatska, 1997. (predavanje, domaća recenzija, sažetak, znanstveni)
CROSBI ID: 371870 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Molecular-cytogenetical characterization of the natural hybrids and polyploids of common onion Alluim cepa L.
Autori
Puizina, Jasna ; Papeš, Dražena
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
Proceedings of Abstacts of the Sixth Congress of Croatian Biologists
/ - , 1997
Skup
The Sixth Congress of Croatian Biologists
Mjesto i datum
Opatija, Hrvatska, 22.09.1997. - 26.09.1997
Vrsta sudjelovanja
Predavanje
Vrsta recenzije
Domaća recenzija
Ključne riječi
triploid; Allium; karyotype; C-banding; genomic in situ hybridization
Sažetak
By application of Feulgen and Giemsa C-banding techniques the hybrid structures of natural diploid and triploid hybrids of common onion Allium cepa were confirmed. Those two forms are under the name shalots traditionally cultivated in the region of South Croatia. For the purpose of identification of the origin of genomes in their karyotypes, technique genomic in situ hybridization was applied. In the diploid karyotype of "Ljutika-talijanka" (2n=2x=16) DNA probes of A. cepa and A. fistulosum completely labelled 8 chromosomes each. DNA probes of A. cepa and Himalayan onion A. roylei Stearn hybridized with the chromosomes of triploid "Ljutika" (2n=3x=24). Each DNA probe labelled one genome (8 chromosomes). DNA probe of A. fistulosum failed to hybridize with the chromosomes of triplod onion. Origin of the third genome remained unresolved, but one can assume it originates from the third as yet unknown Allium species. Comparison of the molecular-cytogenetic characteristics of "Ljutika" and Indian natural triploid "Pran" (2n=3x=24) revealed similar karyotye, highly similar DNA content (2c=43 pg) and identical random amplified polymorphic DNA (RAPD) pattern. With respect to high similarity between domestic "Ljutika" and Indian "Pran" as well as identification of endmic Himalayan species Allium roylei in the karyotype of "Ljutika" we asume that Dalmatian "Ljutika" was introduced probably in ancient times to our region from the region of Central Asia and Far East.
Izvorni jezik
Engleski
