Naslov
Towards the in vitro method for fast evaluation of immunogenic potential of snake venom antigen

Autori
Habjanec, Lidija ; Halassy, Beata ; Brgles, Marija ; Lang Balija, Maja ; Tomašić, Jelka

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
2008 Annual Meeting of the Croatian Immunological Society , Book of Abstracts / - , 2008, 52-52

Skup
2008 Annual Meeting of the Croatian Immunological Society

Mjesto i datum
Šibenik, Hrvatska, 09.10.2008. - 12.10.2008

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
snake venom; AtxA; LD50

Sažetak
V. ammodytes ammodytes venom has been used for active immunisation of animals in antivenom production. We have found recently that the protective efficacy of antisera from venom-immunized rabbits highly correlated with the level of IgGs specific for Ammodytoxin A (AtxA)1. It has also been shown that higher Atx content contributes to higher lethal toxicity of the venom sample2. Also, the venom with higher Atx content induced higher level of anti-AtxA antibodies2. Obviously, the in vitro methods determining Atx content might be predictive of the toxicity and immunogenic potential of venom samples. The aim of this work was to test the two in vitro methods for AtxA content determination: a) recently described chromatographic method2, and b) the novel sandwich ELISA, and to compare results obtained for panel of several different venom samples with their lethal toxicities (LD50) determined by in vivo assay. We also compared the results of the novel assays to the results of several methods currently routinely used for snake venom characterisation (MHD, MND, SDS-PAGE, protein content determination). The results obtained showed that new methods might allow quick and reliable screening of the AtxA-content in the venom sample before using it for the immunisation of experimental animals for antiserum production. 1Halassy et al. Comp Biochem Physiol C 148(2008) 178-183 2Halassy et al. J. Liquid Chromatogr Related Technol 31 (2008) 1-16

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti, Biotehnologija

POVEZANOST RADA