Naslov
Cytotoxic And Genotoxic Potential Of Surface And Waste Waters Using The Allium And Comet Tests

Autori
Radić, Sandra ; Stipaničev, Draženka ; Cvjetko, Petra ; Marijanović Rajčić, Marija ; Širac, Siniša, Pevalek-Kozlina, Branka ; Pavlica, Mirjana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Environmental and Molecular Mutagenesis, vol. 49 / - Danver (MA) : Willey-Blackwell, 2008, 568-568

Skup
EMS 39th Annual Meeting

Mjesto i datum
San José, Portoriko, 18.10.2008. - 24.10.2008

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
genotoxicity; monitoring; Allium; Lemna

Sažetak
Analysis of genotoxicity is of a great importance due to the pollution of the environment by genotoxicants including risk assessment evaluation. In this study the response of the Allium cepa and Lemna minor L. genetic material to the presence of potential cytotoxic and genotoxic substances in surface and wasterwater samples was monitored. Also the suitability of the Allium test and alkaline comet assay as systems for environmental monitoring of surface and wastewaters were investigated. The surface waters were collected at three sampling sites along the river Sava and its confluents while wastewater samples were collected from sewage and industrial effluents near Zagreb, Croatia. Morphological modifications in the A. cepa roots, inhibition of root growth and cell division, aberrant cells in metaphase and anaphase as parameters of Allium test were observed. Tail extent moment as a measure of DNA strand breaks was evaluated by using comet assay on Lemna minor plants. The most polluted water samples caused the inhibition of root growth over 50% (even up to 65%), decrease of mitotic index over 40%, increase of aberrant cells for more than 10 times and increase of tail extent moment for more than 35 times in comparison to control. Obtained data demonstrate the mutagenic activity in some samples of tested waters and suitability of both tests for complex assessment of surface as well as waste waters.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA