Naslov
Standardization of experimental conditions for blood cholinesterase activity measurements

Autori
Reiner, Elsa ; Škrinjarić-Špoljar, Mira ; Simeon-Rudolf, Vera

Vrsta, podvrsta i kategorija rada
Radovi u zbornicima skupova, cjeloviti rad (in extenso), znanstveni

Izvornik
Proceedings of the Chemical and Biological Medical Treatment Symposium-Industry-I, Zagreb-Dubrovnik, 1998 / Bokan, Slavko ; Orehovec, Zvonko ; Price, Barbara - Zagreb : Price, Richard ; Applied Science and Analysis, ASA, 1999, 296-297

Skup
Chemical and Biological Medical Treatment Symposium Industry-I, Zagreb-Dubrovnik

Mjesto i datum
Dubrovnik, Hrvatska; Zagreb, Hrvatska, 25.10.1999. - 31.10.1999

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
acetylcholinesterase; butyrylcholinesterase; cholinesterase inhibitors

Sažetak
We have standardized the Ellman et al. spectrophotometric method with the thiol reagent DTNB under the following conditions: 25°C, 0.1 M phosphate buffer pH=7.4, 1.0 mM acetylthiocholine. Under these conditions, about 92 % whole blood ChE activity corresponds to AChE and 8 % to BChE. The method is applicable over a temperature range between 10 and 40°C. A temperature conversion table has been worked out to convert activities measured at different temperatures. Inhibited blood samples containing unreacted ChE inhibitors were suggested to be stored diluted in 20 mM phosphate buffer pH=5, which did not affect the enzyme activity, but slowed down the rate of further ChE inhibition and the rate of spontaneous reactivation. We support recommendations from the current literature that AChE activities should be expressed per haemoglobin concentration, and we suggest that.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

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