Naslov
Delivery of specific targeted drugs into the cells by TAT-technology

Autori
Grdiša Mira

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
EHRLICH II - 2nd World Conference on Magic Bullets / F. Sorgel - Nürnberg, 2008, 93-93

Skup
EHRLICH II - 2nd World Conference on Magic Bullets

Mjesto i datum
Nürnberg, Njemačka, 03.10.2008. - 05.10.2008

Vrsta sudjelovanja
Pozvano predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
TAT-technology; p27; apoptosis

Sažetak
Background: The importance of drug delivery is pivotal in the wide area of pharmacological research and it has not been solved yet. The main goal of every drug delivery system is the delivery of a precise amount of a drug to the desired location in order to achive the necessary drug concentration in the targeted organ for effective treatment. Proteins and peptides are useful research and therapeutic tools, however their applications are limited because delivery to the desired location is not easily achiveable. Process of protein transduction, using TAT-technology, allows the delivery of drugs and genetic materials inside the cells. This process occurres in a rapid, concentration-dependent fashion that appears to be independent of receptors and transporters. It has a broad implications in experimental systems for regulating intracellular processes and has the potential to be used in the development of new therapeutic strategies for cancer, infectious diseases, and development of vaccines. Hyper proliferation of cancer cells is associated with deregulation of cell cycle progression, which is driven by the activities of CDKs. A key regulator of their activities is protein p27. It has significant role in cancer progression and antitumor drug response. Results: To examine p27 as specific target molecule and its role in tumor cells apoptosis, transduction of TAT-p27, TAT-ptp27 and TAT-N'p27 was performed. It was shown that different forms of TAT-p27 protein can modulate the cell cycle of cultured cell lines and induced apoptosis, depending on the concentration and type of the cells. Also was shown that different signal transduction pathways were involved in induction of apoptosis. Conclusions: Extraxellular p27 could be use for induction of apoptosis in tumor cells. Protein transduction therefore could give an opportunity for delivering of drugs into cells with emphasis on specific target molecules.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA