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Pregled bibliografske jedinice broj: 369299

Structural characterization of PEGylated rHuG-CSF and location of PEG attachment sites

Cindrić, Mario; Čepo, Tina; Galić, Nives; Bukvić-Krajačić, Mirjana; Tomczyk, Nick; Vissers, Johaness P. C.; Bindila, Laura; Peter-Katalinić, Jasna
Structural characterization of PEGylated rHuG-CSF and location of PEG attachment sites // Journal of Pharmaceutical and Biomedical Analysis, 44 (2007), 2; 388-395 doi:10.1016/j.jpba.2007.02.036 (međunarodna recenzija, članak, znanstveni)

CROSBI ID: 369299 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Structural characterization of PEGylated rHuG-CSF and location of PEG attachment sites

Autori
Cindrić, Mario ; Čepo, Tina ; Galić, Nives ; Bukvić-Krajačić, Mirjana ; Tomczyk, Nick ; Vissers, Johaness P. C. ; Bindila, Laura ; Peter-Katalinić, Jasna

Izvornik
Journal of Pharmaceutical and Biomedical Analysis (0731-7085) 44 (2007), 2; 388-395

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Mono-PEGylated rHuG-CSF; LC– MS/MS; MALDI-TOF MS; Lys derivatization; MSE; N-terminal PEG-peptide

Sažetak
Mass spectrometry structural characterization is an essential tool in validating the quality of PEG-rHu-proteins. However, in either case top-down or bottom-up fashion, the interference of high intensity PEG signals on MS detection or detrimental influence of PEG on protein structure, leads to incomplete structural characterization. We propose here a method that permits complete and reliable structural characterization of PEGylated recombinant human granulocyte-colony stimulating factor (rHuG-CSF). The approach includes on-column 2-methoxy-4, 5-dihydro-1H-imidazole derivatization of digested PEG rHuG-CSF and subsequent LC/MS investigation. By comparing the LC/MS retention of derivatized and underivatized digested PEG rHuG-CSF, location of the PEG attachment within rHuG-CSF could be deduced. Besides, the protein sequence coverage and position of the disulfide bridges was fully deducible from the MS data interpretation. Additionally, ultra performance liquid chromatography– mass spectrometry-to-the-E (UPLC– MSE) was introduced for analysis of label-free digested PEG rHuG-CSF here to enable high resolution and mass accuracy of MS detection and facilitate deep structural insights of peptides.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Temeljne medicinske znanosti, Farmacija



POVEZANOST RADA

Projekti:
119-1191342-1083 - Interakcije i dizajn bioaktivnih molekula (Novak, Predrag, MZOS ) ( CroRIS)
006-0000000-3216 - Sinteza, karakterizacija i djelovanje potencijalnih i poznatih ljekovitih tvari (Zorc, Branka, MZOS ) ( CroRIS)
119-1191342-2960 - Elektroliti i koordinacijske reakcije u otopini (Tomišić, Vladislav, MZOS ) ( CroRIS)

Ustanove:
Pliva-Istraživački institut,
Prirodoslovno-matematički fakultet, Zagreb

Profili:

Avatar Url Mirjana Bukvić (autor)

Avatar Url Nives Galić (autor)

Avatar Url Mario Cindrić (autor)

Poveznice na cjeloviti tekst rada:

doi

Citiraj ovu publikaciju:

Cindrić, Mario; Čepo, Tina; Galić, Nives; Bukvić-Krajačić, Mirjana; Tomczyk, Nick; Vissers, Johaness P. C.; Bindila, Laura; Peter-Katalinić, Jasna
Structural characterization of PEGylated rHuG-CSF and location of PEG attachment sites // Journal of Pharmaceutical and Biomedical Analysis, 44 (2007), 2; 388-395 doi:10.1016/j.jpba.2007.02.036 (međunarodna recenzija, članak, znanstveni)
Cindrić, M., Čepo, T., Galić, N., Bukvić-Krajačić, M., Tomczyk, N., Vissers, J., Bindila, L. & Peter-Katalinić, J. (2007) Structural characterization of PEGylated rHuG-CSF and location of PEG attachment sites. Journal of Pharmaceutical and Biomedical Analysis, 44 (2), 388-395 doi:10.1016/j.jpba.2007.02.036.
@article{article, author = {Cindri\'{c}, Mario and \v{C}epo, Tina and Gali\'{c}, Nives and Bukvi\'{c}-Kraja\v{c}i\'{c}, Mirjana and Tomczyk, Nick and Vissers, Johaness P. C. and Bindila, Laura and Peter-Katalini\'{c}, Jasna}, year = {2007}, pages = {388-395}, DOI = {10.1016/j.jpba.2007.02.036}, keywords = {Mono-PEGylated rHuG-CSF, LC and \#8211, MS/MS, MALDI-TOF MS, Lys derivatization, MSE, N-terminal PEG-peptide}, journal = {Journal of Pharmaceutical and Biomedical Analysis}, doi = {10.1016/j.jpba.2007.02.036}, volume = {44}, number = {2}, issn = {0731-7085}, title = {Structural characterization of PEGylated rHuG-CSF and location of PEG attachment sites}, keyword = {Mono-PEGylated rHuG-CSF, LC and \#8211, MS/MS, MALDI-TOF MS, Lys derivatization, MSE, N-terminal PEG-peptide} }
@article{article, author = {Cindri\'{c}, Mario and \v{C}epo, Tina and Gali\'{c}, Nives and Bukvi\'{c}-Kraja\v{c}i\'{c}, Mirjana and Tomczyk, Nick and Vissers, Johaness P. C. and Bindila, Laura and Peter-Katalini\'{c}, Jasna}, year = {2007}, pages = {388-395}, DOI = {10.1016/j.jpba.2007.02.036}, keywords = {Mono-PEGylated rHuG-CSF, LC and \#8211, MS/MS, MALDI-TOF MS, Lys derivatization, MSE, N-terminal PEG-peptide}, journal = {Journal of Pharmaceutical and Biomedical Analysis}, doi = {10.1016/j.jpba.2007.02.036}, volume = {44}, number = {2}, issn = {0731-7085}, title = {Structural characterization of PEGylated rHuG-CSF and location of PEG attachment sites}, keyword = {Mono-PEGylated rHuG-CSF, LC and \#8211, MS/MS, MALDI-TOF MS, Lys derivatization, MSE, N-terminal PEG-peptide} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE

Uključenost u ostale bibliografske baze podataka::


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  • BIOSIS Previews (Biological Abstracts)
  • CA Search (Chemical Abstracts)
  • EMBASE (Excerpta Medica)
  • MEDLINE
  • Scopus, Research alert
  • Cambridge Scientific Abstracts

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