Naslov
Modulators of Calcium Influx Regulate Membrane Excitability in Rat Dorsal Root Ganglion Neurons

Autori
Lirk, Phillip ; Poroli, Mark ; Rigaud, Marcel ; Fuchs, Andreas ; Fillip, Patrick ; Huang, Chun-Yuan ; Ljubković, Marko ; Sapunar, Damir ; Hogan, Quinn

Izvornik
Anesthesia and analgesia (0003-2999) 107 (2008), 2; 673-685

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Voltage-gated calcium ; Spinal nerve ligation ; Action-potential repolarization ; Ca2+-induced ca2+ release ; Primary afferent neurons ; Autotomy-induced changes ; Sensory neurons ; Extracellular calcium ; Hippocampal-neurons ; Sodium-channels

Sažetak
Background: Chronic neuropathic pain resulting from neuronal damage remains difficult to treat, in part, because of incomplete understanding of underlying cellular mechanisms. We have previously shown that inward Ca2+ flux (I-Ca, ) across the sensory neuron plasmalemma is decreased in a rodent model of chronic neuropathic pain, but the direct consequence of this loss of ICa on function of the sensory neuron has not been defined. We therefore examined the extent to which altered membrane properties after nerve injury, especially increased excitability that may contribute to chronic pain, are attributable to diminished Ca2+ entry. Methods: Intracellular microelectrode measurements were obtained from A-type neurons of dorsal root ganglia excised from uninjured rats. Recording conditions were varied to suppress or promote I-Ca while biophysical variables and excitability were determined. Results: Both lowered external bath Ca2+ concentration and blockade of I-Ca with bath cadmium diminished the duration and area of the after-hyperpolarization (AHP), accompanied by decreased current threshold for action potential (AP) initiation and increased repetitive firing during sustained depolarization. Reciprocally, elevated bath Ca2+ increased the AHP and suppressed repetitive firing. Voltage sag during neuronal hyperpolarization, indicative of the cation- nonselective H-current, diminished with decreased bath Ca2+ cadmium application, or chelation of intracellular Ca2+. Additional recordings with selective blockers of I-Ca subtypes showed that N-, P/Q, L-, and R-type currents each contribute to generation of the AHP and that blockade of any of these, and the T-type current, slows the AP upstroke, prolongs the AP duration, and (except for L-type current) decreases the current threshold for AP initiation. Conslusions: Taken together, our findings show that suppression of I-Ca decreases the AHP, reduces the hyperpolarization-induced voltage sag, and increases excitability in sensory neurons, replicating changes that follow peripheral nerve trauma. This Suggests that the loss of I-Ca previously demonstrated in injured sensory neurons contributes to their dysfunction and hyperexcitability, and may lead to neuropathic pain.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA