Naslov
Wistar-Zagreb 5HT rats - a rodent model in serotonin transporter research

Autori
Jernej, Branimir ; Čičin-Šain, Lipa

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstracts of the 13th World Congress on Advances in Oncology and 11th International Symposium on Molecular Medicine ; u: International Journal of Molecular Medicine. Supplement / Spandidos, Demetrios A. - Atena : Spandidos Publications, 2008, S73-S73

Skup
13th World Congress on Advances in Oncology and 11th International Symposium on Molecular Medicine

Mjesto i datum
Hersonissos, Grčka, 09.10.2008. - 11.10.2008

Vrsta sudjelovanja
Pozvano predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Wistar-Zagreb 5HT rat; serotonin; serotonin transporter

Sažetak
Two sublines of rats with constitutional hyperserotonemia / hyposerotonemia were developed by selective breeding for the extreme values of platelet serotonin level. The velocity of platelet serotonin uptake (PSU), the main determinant of PSL, was used as a further, more specific, selection criterion and directed breeding for its extremes resulted in two sublines of rats with constitutional upregulation / down-regulation of platelet 5HT transporter activity, and consequent alterations of entire 5HT homeostasis. These sublines, termed Wistar-Zagreb 5HT rats (WZ-5HT rats), constitute a genetic rodent model in serotonin research. Besides changes in peripheral 5HT homeostasis, high-5HT and low-5HT sublines of WZ-5HT rats also demonstrated changes in central serotonergic mechanisms. Under physiological conditions, neurochemical differences in the 5 HT system between sublines were almost undetectable, but they become clearly evident upon specific pharmacologic challenge, as shown by in vivo microdialysis study. Differential behavioral phenotypes of 5HT sublines in response to various environmental challenges provide further evidence for differences in their brain functioning. Thus, high-5HT rats exhibit enhanced anxiety-like behaviors while depressive-like behavior and higher alcohol intake co-occur in low-5HT animals. Observed functional and behavioural differences between sublines of WZ-5HT rats strongly indicate that the brain serotonergic activity is increased in animals from the high-5HT subline as compared to low-5HT rats. The WZ-5HT rat model may represent an integrative model for serotonin transporter (and serotonin in general) research, incorporating changes at genomic/genetic and phenotypic (neurodevelopmental, structural, biochemical, behavioral etc.) levels and encompassing both central and peripheral 5HT functioning.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti

POVEZANOST RADA