Naslov
The effects of organophosphorous compounds on precursors of human skeletal muscle regeneration

Autori
Marš, Tomaž ; Miš, Katarina ; Pirkmajer, Sergej ; Pegan, Katarina ; Bosak, Anita ; Čalić, Maja ; Kovarik, Zrinka ; Grubič, Zoran

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of Abstracts of the HDBMB 2008, Congress of the Croatian Society of Biochemistry and Molecular Biology with international participation / Strelec, Ivica ; Glavaš-Obrovac, Ljubica - Osijek : Hrvatsko Društvo za Biotehnologiju, 2008, 55-55

ISBN
978-953-95551-2-0

Skup
HDBMB 2008, Congress of the Croatian Society of Biochemistry and Molecular Biology with international participation

Mjesto i datum
Osijek, Hrvatska, 17.09.2008. - 20.09.2008

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Domaća recenzija

Ključne riječi
organophosphorous compounds; muscle regeneration; cholinesterases; stress response

Sažetak
Besides well established effects of organophosphorous (OPs) compounds exerted through inhibition of cholinesterases (ChE), ChE are not the only targets of OPs and various intracellular mechanisms are modified due to direct or indirect OPs actions. Typically met upon the OPs poisoning are myopathies, which might be a consequence of insufficient muscle regeneration. It is therefore important to know whether OPs in any way interfere with the mechanisms involved in muscle regeneration process. The proposed mechanisms of OPs effects on muscle regeneration process include conformational changes of ChE protein which might affect so called noncholinergic acetylcholinesterase (AChE) functions or direct organophosphate binding to some of non-cholinergic molecules. The most important step in the process of muscle regeneration is myoblast proliferation, myoblast fusion and myotube innervation. Our investigations on the effects of OPs on the muscle regeneration were therefore focused on these early stages of muscle regeneration. We studied the effects of OPs on the expression of AChE, heat-shock protein (HSP) 70 and 27, interleukin-6, known for their roles in stress response of the skeletal muscle, the effects on the expression of Na+/K+ATPase protein subunits, which are markers of muscle differentiation. We found that exposure of muscle cells to OPs did not interfere with the processes of myoblast fusion and innervation and AChE expression. In cells exposed to OPs constitutive interleukin-6 secretion was inhibited. The levels of Na+/K+ATPase α 2 subunit and HSP 70 and 27 were higher than in controls. These effects might contribute to the myopatic effects known to accompany OPs poisoning.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Temeljne medicinske znanosti

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