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Pregled bibliografske jedinice broj: 366479

Mathematical modelling of the dehydrogenase catalyzed hexanol oxidation with coenzyme regeneration by NADH oxidase

Vrsalović Presečki, Ana; Vasić-Rački, Đurđa
Mathematical modelling of the dehydrogenase catalyzed hexanol oxidation with coenzyme regeneration by NADH oxidase // Process biochemistry (1991), 44 (2009), 1; 54-61 doi:10.1016/j.procbio.2008.09.007 (međunarodna recenzija, članak, znanstveni)

CROSBI ID: 366479 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Mathematical modelling of the dehydrogenase catalyzed hexanol oxidation with coenzyme regeneration by NADH oxidase

Autori
Vrsalović Presečki, Ana ; Vasić-Rački, Đurđa

Izvornik
Process biochemistry (1991) (1359-5113) 44 (2009), 1; 54-61

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
coenzyme regeneration ; NADH oxidase ; enzyme kinetics ; yeast ; alcohol dehydrogenase ; modelling

Sažetak
The hexanol oxidation catalyzed by alcohol dehydrogenase from baker's yeast (YADH) has been investigated with two different forms of the biocatalyst: the isolated YADH as well as the YADH in the permeabilized whole cells. It was found that in this reaction, equilibrium is shifted to the reduction side. Hence, to increase the conversion it was necessary to regenerate NAD+. For that purpose, enzyme NADH oxidase isolated from Lactobacillus brevis was used. All biocatalysts were kinetically characterized. The overall reaction rate was described by the mathematical model which consisted of kinetics and balance equations. Due to the deactivation of NADH oxidase, only 50-58% hexanol was converted to hexanal in the batch reactor where the hexanol oxidation was catalyzed by isolated YADH. In the case of permeabilized baker's yeast cells, no enzyme deactivation occurred and 100% hexanol conversion in the hexanoic acid was detected.

Izvorni jezik
Engleski

Znanstvena područja
Kemijsko inženjerstvo, Biotehnologija



POVEZANOST RADA

Projekti:
MZOS-125-1252086-2793 - Biokatalizatori i biotransformacije (Vasić-Rački, Đurđa, MZOS ) ( CroRIS)

Ustanove:
Fakultet kemijskog inženjerstva i tehnologije, Zagreb

Profili:

Avatar Url Đurđa Vasić-Rački (autor)

Avatar Url Ana Vrsalović Presečki (autor)

Poveznice na cjeloviti tekst rada:

doi dx.doi.org www.sciencedirect.com

Citiraj ovu publikaciju:

Vrsalović Presečki, Ana; Vasić-Rački, Đurđa
Mathematical modelling of the dehydrogenase catalyzed hexanol oxidation with coenzyme regeneration by NADH oxidase // Process biochemistry (1991), 44 (2009), 1; 54-61 doi:10.1016/j.procbio.2008.09.007 (međunarodna recenzija, članak, znanstveni)
Vrsalović Presečki, A. & Vasić-Rački, Đ. (2009) Mathematical modelling of the dehydrogenase catalyzed hexanol oxidation with coenzyme regeneration by NADH oxidase. Process biochemistry (1991), 44 (1), 54-61 doi:10.1016/j.procbio.2008.09.007.
@article{article, author = {Vrsalovi\'{c} Prese\v{c}ki, Ana and Vasi\'{c}-Ra\v{c}ki, \DJur\dja}, year = {2009}, pages = {54-61}, DOI = {10.1016/j.procbio.2008.09.007}, keywords = {coenzyme regeneration, NADH oxidase, enzyme kinetics, yeast, alcohol dehydrogenase, modelling}, journal = {Process biochemistry (1991)}, doi = {10.1016/j.procbio.2008.09.007}, volume = {44}, number = {1}, issn = {1359-5113}, title = {Mathematical modelling of the dehydrogenase catalyzed hexanol oxidation with coenzyme regeneration by NADH oxidase}, keyword = {coenzyme regeneration, NADH oxidase, enzyme kinetics, yeast, alcohol dehydrogenase, modelling} }
@article{article, author = {Vrsalovi\'{c} Prese\v{c}ki, Ana and Vasi\'{c}-Ra\v{c}ki, \DJur\dja}, year = {2009}, pages = {54-61}, DOI = {10.1016/j.procbio.2008.09.007}, keywords = {coenzyme regeneration, NADH oxidase, enzyme kinetics, yeast, alcohol dehydrogenase, modelling}, journal = {Process biochemistry (1991)}, doi = {10.1016/j.procbio.2008.09.007}, volume = {44}, number = {1}, issn = {1359-5113}, title = {Mathematical modelling of the dehydrogenase catalyzed hexanol oxidation with coenzyme regeneration by NADH oxidase}, keyword = {coenzyme regeneration, NADH oxidase, enzyme kinetics, yeast, alcohol dehydrogenase, modelling} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus

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  • CA Search (Chemical Abstracts)

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