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Pregled bibliografske jedinice broj: 366373

Functional tyrosine residue in the active center of human dipeptidyl peptidase III

Salopek-Sondi, Branka; Vukelić, Bojana; Špoljarić, Jasminka; Šimaga, Šumski; Vujaklija, Dušica; Makarević, Janja; Jajčanin-Jozić, Nina; Abramić, Marija
Functional tyrosine residue in the active center of human dipeptidyl peptidase III // Book of abstracts - 4th Central European Conference Chemistry towards Biology / Gaspari, Zoltan (ur.).
Budimpešta: Hungarian Chemical Society, 2008. str. 60-60 (poster, međunarodna recenzija, sažetak, znanstveni)

CROSBI ID: 366373 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Functional tyrosine residue in the active center of human dipeptidyl peptidase III

Autori
Salopek-Sondi, Branka ; Vukelić, Bojana ; Špoljarić, Jasminka ; Šimaga, Šumski ; Vujaklija, Dušica ; Makarević, Janja ; Jajčanin-Jozić, Nina ; Abramić, Marija

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of abstracts - 4th Central European Conference Chemistry towards Biology / Gaspari, Zoltan - Budimpešta : Hungarian Chemical Society, 2008, 60-60

ISBN
978-963-9319-85-1

Skup
4th Central European Conference Chemistry towards Biology

Mjesto i datum
Dobogókő, Mađarska, 08.09.2008. - 11.09.2008

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Dipeptidyl peptidase III; Metallopeptidase; Tyr318

Sažetak
Human dipeptidyl peptidase III (DPP III) is a member of the metallopeptidase family M49 with an implied role in the pain-modulatory system and endogenous defense against oxidative stress. Apart from active site-zinc ligation, many details of the DPP III (M49 peptidase) mechanism are unknown. We report heterologous expression of human DPP III and the site-directed mutagenesis results which demonstrate a functional role for Tyr318 at this enzyme's active site . The substitution of Tyr318 to Phe decreased kcat by two orders of magnitude without altering the binding affinity of substrate, or of a competitive hydroxamate inhibitor designed to interact with S1 and S2 subsites. The results favor the involvement of the conserved tyrosine in transition state stabilization during the catalytic action of M49 peptidases. References: Salopek-Sondi, B., Vukelić, B., Špoljarić, J., Šimaga, Š., Vujaklija, D., Makarević, J., Jajčanin, N., Abramić, M. (2008): Functional tyrosine residue in the active center of human dipeptidyl peptidase III, Biol. Chem. 389: 163-167.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija



POVEZANOST RADA

Projekti:
098-0982913-2829 - Molekularna regulacija biljnog razvitka (Salopek-Sondi, Branka, MZOS ) ( CroRIS)
098-0982913-2877 - Temeljna molekularno-biološka istraživanja streptomiceta (Vujaklija, Dušica, MZOS ) ( CroRIS)
098-0982904-2912 - Samo-udruživanje u gelovima i sinteza funkcionalnih hibridnih materijala (Frkanec, Leo, MZOS ) ( CroRIS)
098-1191344-2938 - Molekularna enzimologija i proteinske interakcije hidrolaza (Abramić, Marija, MZOS ) ( CroRIS)

Ustanove:
Institut "Ruđer Bošković", Zagreb

Profili:

Avatar Url Šumski Šimaga (autor)

Avatar Url Branka Salopek-Sondi (autor)

Avatar Url Dušica Vujaklija (autor)

Avatar Url Janja Makarević (autor)

Avatar Url Marija Abramić (autor)

Avatar Url Jasminka Špoljarić (autor)

Avatar Url Nina Jajčanin Jozić (autor)

Avatar Url Bojana Vukelić (autor)

Citiraj ovu publikaciju:

Salopek-Sondi, Branka; Vukelić, Bojana; Špoljarić, Jasminka; Šimaga, Šumski; Vujaklija, Dušica; Makarević, Janja; Jajčanin-Jozić, Nina; Abramić, Marija
Functional tyrosine residue in the active center of human dipeptidyl peptidase III // Book of abstracts - 4th Central European Conference Chemistry towards Biology / Gaspari, Zoltan (ur.).
Budimpešta: Hungarian Chemical Society, 2008. str. 60-60 (poster, međunarodna recenzija, sažetak, znanstveni)
Salopek-Sondi, B., Vukelić, B., Špoljarić, J., Šimaga, Š., Vujaklija, D., Makarević, J., Jajčanin-Jozić, N. & Abramić, M. (2008) Functional tyrosine residue in the active center of human dipeptidyl peptidase III. U: Gaspari, Z. (ur.)Book of abstracts - 4th Central European Conference Chemistry towards Biology.
@article{article, author = {Salopek-Sondi, Branka and Vukeli\'{c}, Bojana and \v{S}poljari\'{c}, Jasminka and \v{S}imaga, \v{S}umski and Vujaklija, Du\v{s}ica and Makarevi\'{c}, Janja and Jaj\v{c}anin-Jozi\'{c}, Nina and Abrami\'{c}, Marija}, editor = {Gaspari, Z.}, year = {2008}, pages = {60-60}, keywords = {Dipeptidyl peptidase III, Metallopeptidase, Tyr318}, isbn = {978-963-9319-85-1}, title = {Functional tyrosine residue in the active center of human dipeptidyl peptidase III}, keyword = {Dipeptidyl peptidase III, Metallopeptidase, Tyr318}, publisher = {Hungarian Chemical Society}, publisherplace = {Dobog\'{o}k\H{o}, Ma\djarska} }
@article{article, author = {Salopek-Sondi, Branka and Vukeli\'{c}, Bojana and \v{S}poljari\'{c}, Jasminka and \v{S}imaga, \v{S}umski and Vujaklija, Du\v{s}ica and Makarevi\'{c}, Janja and Jaj\v{c}anin-Jozi\'{c}, Nina and Abrami\'{c}, Marija}, editor = {Gaspari, Z.}, year = {2008}, pages = {60-60}, keywords = {Dipeptidyl peptidase III, Metallopeptidase, Tyr318}, isbn = {978-963-9319-85-1}, title = {Functional tyrosine residue in the active center of human dipeptidyl peptidase III}, keyword = {Dipeptidyl peptidase III, Metallopeptidase, Tyr318}, publisher = {Hungarian Chemical Society}, publisherplace = {Dobog\'{o}k\H{o}, Ma\djarska} }

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