Pregled bibliografske jedinice broj: 366148
In vitro shoot regeneration from immature seeds of Epimedium alpinum induced by thidiazuron and CPPU
In vitro shoot regeneration from immature seeds of Epimedium alpinum induced by thidiazuron and CPPU // Scientia horticulturae, 120 (2009), 3; 406-410 doi:10.1016/j.scienta.2008.11.019 (međunarodna recenzija, članak, znanstveni)
CROSBI ID: 366148 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
In vitro shoot regeneration from immature seeds of Epimedium alpinum induced by thidiazuron and CPPU
Autori
Mihaljević, Snježana ; Vršek, Ines
Izvornik
Scientia horticulturae (0304-4238) 120
(2009), 3;
406-410
Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni
Ključne riječi
barrenwort ; CPPU ; Epimedium alpinum ; immature seed ; organogenesis ; regeneration ; thidiazuron
Sažetak
In vitro propagation of Epimedium alpinum L. was carried out using immature seed explants. The effects of various concentrations of thidiazuron (TDZ) and 1-(2-chloro-4-pyridyl)-3-phenylurea (CPPU), on the induction of organogenic callus was evaluated. Organogenesis occurred most efficiently when explants were transiently exposed (48 hours) to 20 uM CPPU or 80 uM TDZ followed by culture on hormone-free woody-plant medium (WPM). Organogenic callus consisting of white, compact clumps of tissue proliferated slowly on hormone-free WPM. To promote adventitious shoot induction, the effects of different concentrations of 2, 4-dichlorophenoxyacetic acid (2, 4-D) and 6-benzyladenine (BA) were investigated. The highest per cent shoot regeneration, 66.7% of explants, and the maximum mean number of shoots, 2.6 per explant, were obtained on WPM containing 1.1 uM 2, 4-D and 22 uM BA. Shoots were rooted on hormone-free WPM and well-developed plantlets were successfully transferred to soil.
Izvorni jezik
Engleski
Znanstvena područja
Biologija, Poljoprivreda (agronomija)
POVEZANOST RADA
Projekti:
098-0982913-2829 - Molekularna regulacija biljnog razvitka (Salopek-Sondi, Branka, MZOS ) ( CroRIS)
178-0000000-1939 - Hortikultura u odgoju i obrazovanju (Han Doveden, Ines, MZOS ) ( CroRIS)
Ustanove:
Institut "Ruđer Bošković", Zagreb,
Agronomski fakultet, Zagreb
Citiraj ovu publikaciju:
Časopis indeksira:
- Current Contents Connect (CCC)
- Web of Science Core Collection (WoSCC)
- Science Citation Index Expanded (SCI-EXP)
- SCI-EXP, SSCI i/ili A&HCI
- Scopus