Naslov
Genomic instability of the APC gene found in glioblastoma

Autori
Beroš, Vili ; Pećina-Šlaus, Nives ; Houra, Karlo ; Nikuševa-Martić, Tamara

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstracts of the 5th congress of the Croatian Neurosurgical Society and the Joint Congress of Neurological Surgeons ; u: Neurologia Croatica 57 (2008) (S3) / - , 2008, 47-47

Skup
5th Congress of the Croatian Neurosurgical Society (5 ; 2008) ; Congress of Neurological Surgeons

Mjesto i datum
Dubrovnik, Hrvatska, 02.09.2008. - 05.09.2008

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
APC gene; glioblastoma
(gen APC; glioblastom)

Sažetak
The etiology and pathogenesis of tumors of the central nervous system are still inadequately explained. This study analyses tumor suppressor gene— adenomatous polyposis coli (APC) in 28 patients with glioblastoma. Glioblastoma samples were tested for gene instability by PCR/loss of heterozygosity using RFLP method. Two polymorphic markers were used: an Rsa I polymorphic site in exon 11, and an Msp I polymorphic site in exon 15. The results of our analysis for both markers showed allelic loss of the APC gene in 40% of our sample out of 25 heterozygous patients (informativeness 89%). Another 20% of samples demonstrated allelic imbalance of the APC allele in tumor tissue. Altogether, there were 15 samples (60%) demonstrating instability of this tumor suppressor gene. Despite increasing knowledge on glioma biology and genetics, the prognostic tools for glioblastoma still need improvement.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA