Naslov
Free-radical scavenging capacity and antioxidant activity of Berberis croatica Horvat and B. vulgaris L.

Autori
Zovko, Marijana ; Sanković, Krešimir ; Kosalec, Ivan ; Kremer, Dario ; Karlović, Ksenija ; Kalođera, Zdenka

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
7th Joint Meeting of AFERP, ASP, GA, PSE & SIF : Congress Abstracts ; u: Planta Medica 74 (2008) (9) / - , 2008, 972-972

Skup
Joint Meeting of AFERP, ASP, GA, PSE & SIF (7 ; 2008)

Mjesto i datum
Atena, Grčka, 03.08.2008. - 08.08.2008

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
antioxidant activity; Berberis croatica; Berberis vulgaris

Sažetak
Plants of the genus Berberis are commonly used in nutrition and phytomedicine. The purpose of this study was to investigate radical scavenging properties and antioxidant activity of roots, leaves and twigs of Croatian barberry (B. croatica Horvat), an endemic illyric-balcanic species, and compare it to antioxidant activity of common barberry, (B. vulgaris L.). Ethanolic extracts of roots, twigs and leaves of B. croatica and B. vulgaris collected at four locations in Croatia (two locations per species) were investigated. Content of total phenols and flavonols were determined by using Folin Ciocalteu and aluminium chloride method, respectivelly. Antioxidant activity was established using following methods: radical scavenging activity of 2, 2-diphenyl-1-picrylhydrazyl (DPPH) free-radical, reducing power and beta-carotene-linoleic acid assay as well as electron paramagnetic resonance (EPR) spectroscopy. In all the analyses the leaf extracts were found to possess most marked antioxidant activities, not significantly different from positive control butylated hydroxyanisole (BHA) (ANOVA, p>0.05). Activity of twigs and roots extracts was somewhat less pronounced then the activity of leaves extracts. This investigation revealed no statistically significant differences in antioxidant activities between plant species (p>0.05). With the exception of the beta-carotene-linoleic acid assay, antioxidant activity in all the applied assays correlated well with content of total phenols and flavonols (r2>0.83). That suggests that those compounds play an important role in overall antioxidant activity of investigated plant organs.

Izvorni jezik
Engleski

Znanstvena područja
Fizika, Kemija, Farmacija

Napomena
DOI: 10.1055/s-0028-1084132

POVEZANOST RADA