Pretraga

Pretražite po imenu i prezimenu autora, mentora, urednika, prevoditelja

Napredna pretraga

Pregled bibliografske jedinice broj: 36488

Induction of DNA damage and expression of heat shock protein HSP70 by polychlorinated biphenyls in the marine sponge Suberites domuncula Olivi

Schroder, Hans C.; Batel, Renato; Lauenroth, S.; Hassanein, H.M.A.; Lacorn, M.; Simat, T.; Steinhart, H.; Muller, Werner E.G.
Induction of DNA damage and expression of heat shock protein HSP70 by polychlorinated biphenyls in the marine sponge Suberites domuncula Olivi // Journal of experimental marine biology and ecology, 233 (1999), 2; 285-300 (međunarodna recenzija, članak, znanstveni)

CROSBI ID: 36488 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Induction of DNA damage and expression of heat shock protein HSP70 by polychlorinated biphenyls in the marine sponge Suberites domuncula Olivi

Autori
Schroder, Hans C. ; Batel, Renato ; Lauenroth, S. ; Hassanein, H.M.A. ; Lacorn, M. ; Simat, T. ; Steinhart, H. ; Muller, Werner E.G.

Izvornik
Journal of experimental marine biology and ecology (0022-0981) 233 (1999), 2; 285-300

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
DNA damage; heat shock protein hsp70; polychlorinated biphenyls; environmental stress

Sažetak
The effects of different polychlorinated biphenyls (PCBs) including toxic coplanar non-ortho (PCB77) and non-planar mono-ortho (PCB118) and di-ortho (PCB153) congeners on the extent of DNA damage as well as on the expression of heat shock protein 70 (HSP70) were investigated in the marine sponge Suberites domuncula Olivi (Porifera; Demospongiae). A time-dependent increase in the number of DNA single-strand breaks, expressed as strand scission factor (SSF), was found after injection of a single dose of 25 mu g of PCB 118 or PCB 153 per gram wet mass of S. domuncula, using Fast Micromethod assay, which is based on the unwinding of DNA under alkaline conditions. The number of strand breaks induced by PCB153 was about two-fold higher than that observed for PCB118 after an incubation period of six days. Unexpectedly, a strong reduction in the rate of DNA unwinding compared to untreated control, that could be indicative for the formation of DNA crosslinks, was found following treatment of S. domuncula with PCB77, which is, in vertebrate systems, the most toxic among the PCB congeners studied. The three selected PCB congeners are able to induce the expression of both "constitutive" (HSP73; M-r 73 kDa) and "inducible" (HSP75; M-r 75 kDa) HSP70 proteins. The expression of HSP75 was markedly lower than that observed for HSP73, and varied considerably among different sponge individuals exposed to PCB77. The results in this work demonstrate that: (1) marine sponges (S. domuncula) respond to distinct PCB congeners by induction of different degrees of DNA damage, and (2) expression of HSP is a potential (but not specific) biomarker for PCB exposure in sponges.

Izvorni jezik
Engleski

Znanstvena područja
Biologija



POVEZANOST RADA

Projekti:
00981306

Ustanove:
Institut "Ruđer Bošković", Zagreb

Profili:

Avatar Url Renato Batel (autor)

Citiraj ovu publikaciju:

Schroder, Hans C.; Batel, Renato; Lauenroth, S.; Hassanein, H.M.A.; Lacorn, M.; Simat, T.; Steinhart, H.; Muller, Werner E.G.
Induction of DNA damage and expression of heat shock protein HSP70 by polychlorinated biphenyls in the marine sponge Suberites domuncula Olivi // Journal of experimental marine biology and ecology, 233 (1999), 2; 285-300 (međunarodna recenzija, članak, znanstveni)
Schroder, H., Batel, R., Lauenroth, S., Hassanein, H., Lacorn, M., Simat, T., Steinhart, H. & Muller, W. (1999) Induction of DNA damage and expression of heat shock protein HSP70 by polychlorinated biphenyls in the marine sponge Suberites domuncula Olivi. Journal of experimental marine biology and ecology, 233 (2), 285-300.
@article{article, author = {Schroder, Hans C. and Batel, Renato and Lauenroth, S. and Hassanein, H.M.A. and Lacorn, M. and Simat, T. and Steinhart, H. and Muller, Werner E.G.}, year = {1999}, pages = {285-300}, keywords = {DNA damage, heat shock protein hsp70, polychlorinated biphenyls, environmental stress}, journal = {Journal of experimental marine biology and ecology}, volume = {233}, number = {2}, issn = {0022-0981}, title = {Induction of DNA damage and expression of heat shock protein HSP70 by polychlorinated biphenyls in the marine sponge Suberites domuncula Olivi}, keyword = {DNA damage, heat shock protein hsp70, polychlorinated biphenyls, environmental stress} }
@article{article, author = {Schroder, Hans C. and Batel, Renato and Lauenroth, S. and Hassanein, H.M.A. and Lacorn, M. and Simat, T. and Steinhart, H. and Muller, Werner E.G.}, year = {1999}, pages = {285-300}, keywords = {DNA damage, heat shock protein hsp70, polychlorinated biphenyls, environmental stress}, journal = {Journal of experimental marine biology and ecology}, volume = {233}, number = {2}, issn = {0022-0981}, title = {Induction of DNA damage and expression of heat shock protein HSP70 by polychlorinated biphenyls in the marine sponge Suberites domuncula Olivi}, keyword = {DNA damage, heat shock protein hsp70, polychlorinated biphenyls, environmental stress} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus

Podijeli:





Contrast
Increase Font
Decrease Font
Dyslexic Font
CROSBI koristi kolačiće (cookies) kako bi poboljšao funkcionalnost stranice.