Naslov
Mathematical modeling of amylase catalyzed starch hydrolysis

Autori
Vrsalović Presečki, Ana ; Findrik, Zvjezdana ; Vasić-Rački, Đurđa

Vrsta, podvrsta i kategorija rada
Radovi u zbornicima skupova, cjeloviti rad (in extenso), znanstveni

Izvornik
18th International Congress of Chemical and Process Engineering, Summaries 1, Reaction Engineering: Full text on CD-ROM / Jan Novosad - Prag : Process Engineering Publisher, 2008, 1-8

ISBN
978-80-02-02048-6

Skup
18th International Congress of Chemical and Process Engineering

Mjesto i datum
Prag, Češka Republika, 24.08.2008. - 28.08.2008

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
starch hydrolysis ; amylase ; modeling

Sažetak
The first step of starch hydrolysis, i.e. liquefaction process has been studied in this work. Two commercial α -amylases from Bacilllus licheniformis, known as Termamyl and Liquozyme have been used for this purpose. pH and temperature dependence of both enzymes activity has been examined. Using the starch as the substrate, the kinetics of both enzymes has been determined at optimal pH and temperature (pH 7, T = 80 °C). Impact of glucose and maltose to the initial reaction rate was also studied. All kinetic data were collected by the initial reaction rate method. Kinetics of both enzymes was described by Michaelis-Menten kinetics with uncompetitive product inhibition. With the estimated kinetic parameters, mathematical models were developed and validated in the repetitive batch and fed-batch reactor. Significant enzyme deactivation was noticed in the starch hydrolysis catalyzed by Termamyl. By the action of Termamyl, glucose and maltose yield was 7 % and 12 % respectively. In the hydrolysis catalyzed by Liquozyme the yield of 16 % on glucose and of 22 % on maltose was observed

Izvorni jezik
Engleski

Znanstvena područja
Kemijsko inženjerstvo, Biotehnologija

POVEZANOST RADA