Pregled bibliografske jedinice broj: 364213
Stress-induced somatic embryogenesis in pumpkin (C. pepo L.)
Stress-induced somatic embryogenesis in pumpkin (C. pepo L.) // Physiologia Plantarum, special issue, 133 (3) / Vaughan, Hurry (ur.).
Oxford: Wiley-Blackwell, 2008. str. P10-036 (poster, međunarodna recenzija, sažetak, znanstveni)
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Naslov
Stress-induced somatic embryogenesis in pumpkin (C. pepo L.)
Autori
Mihaljević, Snježana ; Radić, Sandra ; Bauer, Nataša ; Leljak Levanić, Dunja ; Jelaska, Sibila
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
Physiologia Plantarum, special issue, 133 (3)
/ Vaughan, Hurry - Oxford : Wiley-Blackwell, 2008, P10-036
Skup
XVI Congress of the Federation of European Societies of Plant Biology (FESPB)
Mjesto i datum
Tampere, Finska, 17.08.2008. - 22.08.2008
Vrsta sudjelovanja
Poster
Vrsta recenzije
Međunarodna recenzija
Ključne riječi
Cucurbita pepo L.; esteraze; fenilalanin-amonij-lijaza; nedostatak dušika; somatska embriogeneza; peroksidaze
(Cucurbita pepo L.; esterases; nitrogen deficiency; phenylalanine ammonia-lyase; peroxidases; somatic embryogenesis)
Sažetak
Proembryo production in pumpkin can be induced by low concentrations of NH4Cl as a sole source of nitrogen, or by 2, 4-D. Subsequent addition of nitrogen in the first case, and removal of auxin in the second, stimulate more advanced stages of embryo development. Proembryogenic cells from the two systems showed differences in growth parameters and patterns of callose deposition. The latter disappeared when the tissue was transferred onto a medium that enables late embryo development. The activities of soluble peroxidase (EC 1.11.1.7), esterase and phenylalanine ammonia-lyase, enzymes shown to be markers of stress response and embryo development, were the highest in tissue grown in NH4+-only medium which reflects highly proembryogenic state of the culture. Enzymatic activities decreased after buffering of the medium with MES, and additionally decreased after re-supply of different forms of nitrogen or in medium with 2, 4-D. The effect of L-glutamine on somatic embryogenesis was stronger than that of nitrate. The role of stress during early somatic embryogenesis in pumpkin will be discussed.
Izvorni jezik
Engleski
Znanstvena područja
Biologija
POVEZANOST RADA
Projekti:
098-0982913-2829 - Molekularna regulacija biljnog razvitka (Salopek-Sondi, Branka, MZOS ) ( CroRIS)
119-1191196-1202 - Molekularni mehanizmi odgovora hrvatskih endemičnih i rijetkih biljaka na stres (Pevalek-Kozlina, Branka, MZOS ) ( CroRIS)
119-1191196-1225 - Faktori i biljezi embriogenog potencijala i fenolnog metabolizma u biljaka (Jelaska, Sibila, MZOS ) ( CroRIS)
Ustanove:
Institut "Ruđer Bošković", Zagreb,
Prirodoslovno-matematički fakultet, Zagreb
Profili:
Snježana Mihaljević
(autor)
Dunja Leljak-Levanić
(autor)
Nataša Bauer
(autor)
Sibila Jelaska
(autor)
Sandra Radić Brkanac
(autor)
Citiraj ovu publikaciju:
Časopis indeksira:
- Current Contents Connect (CCC)
- Web of Science Core Collection (WoSCC)
- Science Citation Index Expanded (SCI-EXP)
- SCI-EXP, SSCI i/ili A&HCI
- Scopus
- MEDLINE