Naslov
Carboxypeptidase II, Catalase, and Polyphenol oxidase Activities as Potential Indicators of Wheat Seeds Infestation by Sitophilus Granarius

Autori
Strelec, Ivica ; Mrša, Vladimir ; Ugarčić-Hardi, Žaneta

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of Abstracts of the 13th ICC Cereal and Bread Congress / Collar, Concha - Madrid : Spanish Association for Cereal Science and Technology, 2008, 170-170

ISBN
978-84-612-4517-8

Skup
13th ICC Cereal and Bread Congress

Mjesto i datum
Madrid, Španjolska, 15.06.2008. - 18.06.2008

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
pšenica; oksidoreduktaze; peptidaze; zaraza zrna; žitni žižak (Sitophilus granarius)
(wheat seeds; oxidoreductases; peptidases; seed infestation; Sitophilus granarius)

Sažetak
Approximately 10 to 30 % of produced grains worldwide are lost every year during storage due to insect damage. Among them, Sitophilus granarius and Rhyzoperta dominica are two of the most destructive pests of stored wheat grains, whose immature stages develop inside kernels causing weight loss, fungal growth and quality loss. Detection of these insects in stored grains is essential to prevent economic losses during storage and production of contaminated products, as well as to ensure pesticide use only when necessary. Widely accepted methods for the detection of insects in grains rely on traditional methods of sampling, followed by sieving and visual detection, while internal infestation by larvae and hidden insects include staining of egg plugs, flotation of damaged kernel, counting insect fragments or infested seeds, or X-ray analysis. Since some of these methods are time-consuming, some expensive, and some could be subjective and tedious, alternative methods for detection of internal infestation should be found. The objective of this research was to examine possibilities of using oxidoreductive and proteolytic enzyme activity measurement for the detection of internal seed infestation by Sitophilus granarius. Infested and non-infested wheat seed samples of Žitarka variety were examined for activities of glutathione reductase (GR), catalase (CAT), guaiacol peroxidase (POX), polyphenol oxidase (PPO), lipooxygenase (LOX), aminopeptidases preferentially splitting N-terminal amino acids of arginine and phenylalanine (Arg-AP, Phe-AP), carboxypeptidase II (CP-II) and aspartic protease. Results showed that internal wheat seeds infestation could be recognized by increase or decrease of enzyme activities as follows: (a) increase ; CAT > PPO > Aspartic protease > GR > POX ; (b) decrease ; CP-II > LOX. Enzyme activity differences between non-infested and infested wheat seeds indicate that carboxypeptidase II (91 % activity decrease), catalase (172 % activity increase), and polyphenol oxidase (145 % activity increase) are potential indicators of wheat seed infestation by Sitophilus granarius.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija, Prehrambena tehnologija

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