Naslov
Genetic heterogeneity of L-Zagreb mumps virus vaccine strain

Autori
Košutić-Gulija, Tanja ; Forčić, Dubravko ; Šantak, Maja ; Ramljak, Ana ; Mateljak-Lukačević, Sanja ; Mažuran, Renata

Izvornik
Virology Journal (1743-422X) 5 (2008); 79-1

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
mumps; quasispecies; genetic heterogeneity; L-Zagreb strain

Sažetak
The most often used mumps vaccine strains Jeryl Lynn (JL), RIT4385, Urabe-AM9, L-Zagreb and L-3 differ in immunogenicity and reactogenicity. Previous analyses showed that JL, Urabe-AM9 and L-3 are genetically heterogeneous. We identified the heterogeneity of L-Zagreb throughout the entire genome. Two major variants were defined: variant A being identical to the consensus sequence of viral seeds and vaccine(s) and variant B which differs from variant A in three nucleotide positions. The difference between viral variants in L-Zagreb strain is insufficient for distinct viral strains to be defined. We demonstrated that proportion of variants in L-Zagreb viral population depends on cell substrate used for viral replication in vitro and in vivo. CL-Zagreb strain should be considered as a single strain composed of at least two variant viral genomes.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA