Naslov
Anti-oxidative/anti-inflammatory paraoxonase activity and lipid alterations after total splenectomy and autologous spleen transplantation in pigs

Autori
Turk, Romana ; Vnuk, Dražen ; Svetina, Ante ; Flegar-Meštrić, Zlata ; Brkljača Bottegaro, Nika ; Juretić, Dubravka

Izvornik
Veterinarski arhiv (0372-5480) 79 (2009), 1; 1-10

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
paraoxonase ; serum lipids ; splenectomy ; spleen autotransplantation ; surgical trauma ; inflammation

Sažetak
The objective of this study was to investigate serum PON1 activity and lipid and lipoprotein status after total splenectomy and autologous spleen transplantation in pigs in order to assess the effect of surgical stress and trauma on PON1 activity and lipid metabolism. Nineteen piglets used in the experiment were randomly divided into three groups: sham-operation with spleens intact (n=6), total splenectomy (n=6), and splenic autotransplantation (n=7) with small fragments of the spleen autotransplanted into the greater omentum. The blood samples were taken just before surgery and on 1st, 5th, 12th, 26th and 40th day postoperatively. PON1 activity, total cholesterol and HDL-C were assayed in the sera. PON1 activity was significantly decreased postoperatively in splenectomized pigs (on days 5, 12 and 40) and in autotransplantated pigs (on days 5, 12, 26 and 40) while there were no PON1 changes in the sham- operated. In sham-operated pigs, total cholesterol was significantly decreased only on 12th day postoperatively, while HDL-C was significantly decreased postoperatively on 1st, 5th, 12th, 26th and 40th day with the minimum value on 12th day. In splenectomized pigs total cholesterol was significantly decreased on 5th, 12th, 26th and 40th day postoperatively, while HDL-C was decreased as well on 1st, 5th, 12th and 40th. In pigs with autologous splenic transplants, total cholesterol was significantly decreased on 12th, 26th and 40th day, while there was no difference in HDL-C. Results demonstrated decreased PON1 activity and alterations in the concentrations of total cholesterol and HDL-C after surgery due to surgical trauma and post-surgical inflammatory response.

Izvorni jezik
Engleski

Znanstvena područja
Veterinarska medicina

POVEZANOST RADA