Naslov
Influence of interleukin-8 and interleukin-10 on sporadic colon cancer development and progression

Autori
Čačev, Tamara ; Radošević, Senka ; Križanac, Šimun ; Kapitanović, Sanja

Izvornik
Carcinogenesis (0143-3334) 29 (2008), 8; 1572-1580

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
interleukin-8 ; interleukin-10 ; polymorphism ; mRNA expression ; immunohistochemistry ; colon cancer

Sažetak
Cytokines produced in the tumour microenvironment have an important role in cancer pathogenesis. Altered cytokine expression may result in increased susceptibility to and/or poor prognosis in certain cancers. Therefore the aim of this study was to investigate the influence of interleukin-8 and interleukin-10 on sporadic colon cancer development and progression. In our study, a statistically significant increase in IL-8 mRNA expression and decrease in IL-10 mRNA expression in tumour tissue compared to normal mucous tissue was observed (p=0.003 ; p=1.3x10-9. No association was found between IL-8 -251A/T genotypes and IL-8 mRNA expression in tumour and corresponding normal mucous tissue, as well as susceptibility to sporadic colon cancer. Positive immunohistochemical IL-8 staining was more frequent in moderately and poorly differentiated tumours compared to well differentiated tumours (p=0.024). Finally, interleukin-8 significantly stimulated invasion of HT-29 cells in vitro (p= 0.000172). Significant association of IL-10 -1082 A/G, -819 T/C and -592 A/C genotypes and IL-10 mRNA expression in tumour tissue was observed (p=0.022 ; p=0.013 ; p=0.02). Significant association of -819 T/C and -592 A/C genotypes and IL-10 mRNA expression in corresponding normal mucous tissue was observed (p=0.01 ; p=0.04), as well. IL-10 SNP promoter genotypes associated with low IL-10 mRNA expression (-819 TT ; -592 AA) were also associated with increased risk of sporadic colon cancer compared to high expression genotypes (5.53, 95% CI, 1.53-20.1 ; 4.07, 95% CI, 1.28-12.96). Positive IL-10 immunohistochemical reaction was more frequent in well- and moderately differentiated tumours compared to poorly differentiated tumours (p=0.036). In Dukes’ C tumours positive IL-10 immunohistochemical reaction was less frequent compared to Dukes’ A and B tumours (p=0.023). Taken together our results point to possible tumour promoting role of interleukin-8 and potential protective role of interleukin-10 in sporadic colon cancer.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA