Naslov
Purification and properties of extracellular lipase from Streptomyces rimosus

Autori
Abramić, Marija ; Leščić, Ivana ; Korica, Tamara ; Vitale, Ljubinka ; Saenger, Wolfram ; Pigac, Jasenka

Izvornik
Enzyme and Microbial Technology (0141-0229) 25 (1999), 6; 522-529

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Streptomyces rimosus; bacterial; extracellular; lipase

Sažetak
An extracellular lipase of Streptomyces rimosus R6-554W was isolated from culture filtrate by column chromatography using diethylaminoethyl-cellulose, carboxymethyl-cellulose, hydroxylapatite, Mono S (fast protein liquid chromatography), and Sephadex G-75. It was shown to be a monomeric, basic protein (Mr=27500, pI=8.45), active toward triolein and p-nitrophenyl esters, with preferrence for those with medium size (C8-C12) acyl chain length. Interfacial activation was observed with p-nitophenyl butyrate as substrate. The lipase was most active at 50-60°C and in alkaline conditions around pH 9-10, with p-nitrophenyl palmitate as substrate. It showed high stability at a broad pH range of 4-10 and was fairly thermostable. Dithiothreitol moderately inactivated the enzyme. Phenylmethylsulfonyl fluoride partly inhibited lipase only when added during the hydrolytic reaction.

Izvorni jezik
Engleski

Znanstvena područja
Kemija

POVEZANOST RADA