Naslov
Mammalian Genome Recombineering: Yeast, Still a Helper Microorganism of Choice?

Autori
Vrančić, Mila ; Gregorić, Sandra ; Paravić Radičević, Andrea ; Gjuračić, Krešimir

Izvornik
Food technology and biotechnology (1330-9862) 46 (2008), 3; 237-251

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, pregledni rad, znanstveni

Ključne riječi
yeast ; mammalian genome ; homologous recombination ; recombineering

Sažetak
Functional studies of complex mammalian genomes have been revolutionized by the development of the recombineering methodology. Recombineering represents the sum of in vivo recombinant DNA techniques used for the production and manipulation of targeting vectors by the process of homologous recombination within the host microorganism. Although this methodology was initially developed in yeast, the term recombineering was coined after successful introduction of similar techniques in bacterial cells. Since then, due to simplicity of amplification, manipulation and purification of mammalian targeting vectors, Escherichia coli has become the dominant helper microorganism in functional genomics studies. However, some types of experiments in functional genomics still employ yeast as a unique host for the manipulation of megabase-sized mammalian genomic regions.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Biotehnologija

POVEZANOST RADA