Naslov
Phorbol myristate acetate induces ruffling of the acrosome of human sperm

Autori
Liu, De Yi ; Martić, Mirjana ; Grković, Ivica ; Garrett, Claire ; Dunlop, Marjorie E. ; Baker, H. W. Gordon

Izvornik
Fertility and sterility (0015-0282) 78 (2002), 1; 128-136

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Protein kinase C; Actin; Acrosomal ruffing; Human acrosome reaction

Sažetak
To determine the effect of phorbol myristate acetate (PMA) on human acrosome morphology and the acrosome reaction. DESIGN: Controlled experiments on sperm and unfertilized oocytes from volunteers. SETTING: Academic research and teaching tertiary hospital. PATIENT(S): Sperm samples were from normospermic men and unfertilized oocytes from IVF patients. MAIN OUTCOME MEASURE(S): Acrosome morphology was assessed by using transmission and scanning electron microscopy. The acrosome reaction was assessed by using fluorescein-labeled Pisum sativum agglutinin. RESULT(S): PMA induced acrosome ruffling, indicated by a marked wavy appearance. A significant correlation was found between PMA-induced ruffling and PMA enhancement of the zona pellucida-induced acrosome reaction. Protein kinase C inhibitors bisindolylmalemide I and sangivamycin had no effect on PMA-induced acrosomal ruffling, but actin polymerization inhibitors cytochalasin B and cytochalasin D significantly decreased PMA-induced acrosomal ruffling. In contrast, bisindolylmalemide I, sangivamycin, cytochalasin B, and cytochalasin D significantly decreased both the zona pellucida-induced acrosome reaction and the PMA enhancement of the zona pellucida-induced acrosome reaction. CONCLUSION(S): PMA-induced acrosomal ruffling involves actin polymerization, possibly independent of conventional protein kinase C. Acrosomal ruffling is involved in the PMA augmentation of the zona pellucida-induced acrosome reaction.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA