Naslov
Analysis of reference materials for serum copper and zinc by flame-AAS

Autori
Pizent, Alica ; Telišman, Spomenka

Izvornik
Atomic Spectroscopy (0195-5373) 17 (1996), 2; 88-91

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Atomic Absorption Spectroscopy; plasma; spectrometry

Sažetak
A flame-AAS method was evaluated for its analytical accuracy in the determination of scrum copper and serum zinc in commercially available reference materials: Cation-Cal. Seronorm and Contox for Cu and Zn, and Precilip for Cu. The method includes dilution of serum (1+9) with a water solution of 6% (v/v) 1-butanol and calibration with standards containing a mixture of Cu and Zn in 0.5% (v/v) HCl, diluted in the same way. The detection limits (3 sigma) of the method were 1.27 micrograms/L of Cu and 0.78 micrograms/L of Zn, the day-to-day precision (coefficients of variation) of duplicate determinations in 152 human serum samples were in the range 1.7-4.5% at 831-1772 micrograms/L of Cu and 1.2-3.9% at 601-1245 micrograms/L of Zn and the recoveries (mean+/-SD) were 100.2+/-1.1% of Cu and 99.6+/-0.9% of Zn. The results obtained for three out of the four reference materials were in good agreement with the certified values for both Cu and Zn. On the other hand, the precision and the accuracy of the Cu and Zn results obtained for Contox were poor, probably due to the nonhomogeneity of this lyophilized reference material following its reconstitution with water, and possibly unreliable certified values.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Javno zdravstvo i zdravstvena zaštita

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