Naslov
The ability of lymphocytes from patients with chronic lymphocytic leukemia (CLL) to synthesize in vitro IgM and IgG spontaneously or after incubation with LPS, rIFN-alpha and PWM

Autori
Poljak, Ljiljana ; Šimaga, Šumski ; Vitale, Branko

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Periodicum biologorum/Societas Scientiarum Naturalium Croatica / - Zagreb, 1990, 33/W1 9-33/W1 9

Skup
Alps-Adria Immunology and Allergology Meeting 1990

Mjesto i datum
Opatija, Hrvatska, 14.10.1990. - 17.10.1990

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
Chronic lymphocytic leukemia; IgM; IgG; LPS; rIFN-alpha; PWM

Sažetak
It is general accepted that both B- and T- lymphocyte defects are responsible for frequently observed hypogammaglobulinemia in CLL patients. In this study we have measured the ability of cultivated CLL B-lymphocytes to synthesize IgM or IgG spontaneously or after stimulation with LPS, rIFN-alpha or PWM. Among 30 CLL patients the spontaneous IgM and/or IgG production varied in a wide range from 0 to 500 and 880 ng/ml respectively. LPS alone or in combination with rIFN-alpha stimulated proliferation of B- lymphocytes, accompanied only sporadically by their differentiation into IgM but never into IgG producing cells. On the other hand PWM stimulated both proliferation and differentiation of B- lymphocytes, but again preferentially into IgM secreting cells. These data suggest that CLL B- lymphocytes upon in vitro stimulation might, either proliferate and/or differentiate or do not respond at all depending on the level of their differentiation block. The type of reaction most probably reflects intraclonal heterogeneity among defective CLL B-lymphocytes.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija, Temeljne medicinske znanosti

POVEZANOST RADA