Naslov
Vascular endothelial growth factor polymorphisms -1154 G/A and -460 C/T are not associated with VEGF mRNA expression and susceptibility to sporadic colon cancer

Autori
Čačev, Tamara ; Lončar, Božo ; Seiwerth, Sven ; Spaventi, Šime ; Kapitanović, Sanja

Izvornik
DNA and Cell Biology (1044-5498) 27 (2008), 10; 569-574

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
VEGF; SNP; mRNA expression; colon cancer

Sažetak
Vascular endothelial growth factor is important mediator of angiogenesis and its expression in colorectal tumours is related to tumour progression. VEGF expression has been detected in normal mucosa, primary colon cancers, as well as metastatic tumours and patients with low VEGF expression have a better survival rate. In addition anti-VEGF monoclonal antibody improves overall survival when used in combination with existing metastatic colorectal cancer therapy. Therefore prediction of VEGF production based on individual genetic background might be important for predicting the course of the disease and the efficacy of anti-cancer treatment. Number of studies evaluating the influence of VEGF polymorphisms on cancer susceptibility is growing, however their results are often conflicting. In addition, these studies are rarely accompanied with the expression analysis examining the influence of these polymorphisms on mRNA expression in tumour tissue. In this study, we have examined the influence of VEGF polymorphisms -1154 G/A and -460 C/T on VEGF mRNA expression and susceptibility to sporadic colon cancer by real-time PCR-SNP and mRNA expression analysis .The study included population control group consisting of 160 unrelated volunteers and a group of 160 patients with sporadic colon cancer. According to our results -1154 G/A and -460 C/T do not influence VEGF mRNA expression in colorectal tumours and susceptibility to sporadic colon cancer although the role of other polymorphisms can not be excluded.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA