Naslov
Properties and regulation of pyrimidine-catabolizing enzymes in E.coli

Autori
Šimaga, Šumski ; Kos, Erika

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstracts / - Jeruzalem, 1980, 153/C2-P23

Skup
13th FEBS Meeting

Mjesto i datum
Jeruzalem, Izrael, 24.08.1980. - 29.08.1980

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
Escherichia coli; pyrimidine catabolism

Sažetak
Previous studies from this laboratory have shown that in bacteria E.coli by whitdrawal of ammonium ions from glucose minimal media, an enzymatic system is induced by which exogenous thymine and uracil are catabolised with the release of C-2 as CO2. In the present investigations the response of the catabolic system to various structural analogues and metabolic inhibitors, as well as the control role of glutamine synthetase have been studied in some mutants of E.coli K12 and the following observed: (1) Catabolism of both bases is inhibited by 5-halo uracils, 5-aminouracil, 6-aminouracil, while dihydrouracil and barbituric acid are without effect. (2) The catabolic system is oxygen dependent and sensitive to N-ethylmaleimide, dinitrophenol, KCN and azide. (3) The inhibitory effect of diazosulfanilic acid is less pronounced on uracil degradation than on uracil transport occuring through mediation of uracil phosphoribosyltransferase. (4) Mutants deficient in glutamine synthetase are not able to induce pyrimidine degrading enzyme(s) indicating that glutamine synthetase serves as positive control factor in the synthesis of catabolic enzyme(s).

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija

POVEZANOST RADA