Naslov
Biotechnologist’ s View of the Yeast Cell Wall

Autori
Stuparević, Igor ; Teparić, Renata ; Mrša, Vladimir

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Skup
35th Annual Conference on Yeasts

Mjesto i datum
Smolenice, Slovačka, 16.05.2007. - 18.05.2007

Vrsta sudjelovanja
Pozvano predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
yeast; cell wall; yeast cell wall proteins

Sažetak
Also as a cellular structure by which the cell corresponds with its surrounding. Budding yeast wall has a bilayered structure. The inner layer consists mainly of a network of linear ß-1, 3-glucan, and the outer layer is composed of an unusually high number of mannoproteins. Yeasts have evolved three different ways of attaching proteins to the polysaccharide network. Some proteins are bound to ß-1, 3-glucan noncovalently (Scw – soluble cell wall proteins ; extracted by hot SDS), while others are attached covalently (Ccw – covalently linked cell wall proteins ; extracted by glucanases) either through glycosylphosphatidyl inositol (GPI) anchor and ß-1, 6-glucan, or directly to ß-1, 3-glucan by an alkali labile ester linkage between the γ -carboxyl groups of glutamic acid and hydroxyl groups of glucoses (Pir – proteins with internal repeats ; extracted by mild alkalis). The exact physiological roles for most cell wall proteins are still unknown. Molecular mechanisms for incorporation of different groups of wall proteins, as well as the potential use of these mechanisms for cell surface engineering will be reviewed.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija

POVEZANOST RADA