Pregled bibliografske jedinice broj: 35073
Transformation with sequences from an AUD region in Streptomyces rimosus R6 induces DNA rearrangements
Transformation with sequences from an AUD region in Streptomyces rimosus R6 induces DNA rearrangements // 8th International Symposium on the Genetics of Industrial Microorganisms Program and Abstracts / Cohen, G. ; Aharonovitz, Y. (ur.).
Jeruzalem: KENES - Organizers of Congresses and Tour Operators, 1998. (poster, međunarodna recenzija, sažetak, znanstveni)
CROSBI ID: 35073 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Transformation with sequences from an AUD region in Streptomyces rimosus R6 induces DNA rearrangements
Autori
Perić, Nataša ; Petković, Hrvoje ; Toman, Zora ; Pigac, Jasenka ; Paravić, Andrea ; Cullum, John ; Hunter, S. Iain ; Hranueli, Daslav
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
8th International Symposium on the Genetics of Industrial Microorganisms
Program and Abstracts
/ Cohen, G. ; Aharonovitz, Y. - Jeruzalem : KENES - Organizers of Congresses and Tour Operators, 1998
Skup
8th International Symposium on the Genetics of Industrial Microorganisms
Mjesto i datum
Jeruzalem, Izrael, 28.06.1998. - 02.07.1998
Vrsta sudjelovanja
Poster
Vrsta recenzije
Međunarodna recenzija
Ključne riječi
Streptomyces; amplifiable unit of DNA; deletion; amplification
Sažetak
The oxytetracycline (OTC) gene cluster of S. rimosus R6 lies about 600 kb from one end of the linear chromosome in an amplifiable unit of DNA (AUD) (Pandza et al., Microbiol. 143, 1493, 1997). Constructs were designed to introduce disruptions or deletions into the chromosomal OTC cluster by homologous recombination. However, in nearly all cases (102 of 103 analysed) the transformants did not show the expected structure; they carried amplifications of the introduced genes and surrounding sequences or had deleted part or all of the OTC cluster. It is likely that these problems arise from the chromosomal location of the cluster. Deletions of about 750 kb in size occur that remove all the sequences from the chromosome end through the OTC cluster. The AUD gives rise to an amplified DNA sequence (ADS) of 250 kb which includes the OTC cluster. This is accompanied by deletion of distal sequences and probably leads to the replacement of the chromosome end by the ADS as suggested in S. lividans (Rauland et al., Mol. Gen. Genet. 246, 37, 1995). Thus, DNA rearrangements appear to be highly stimulated by integration of vectors carrying sequences from the AUD region.
Izvorni jezik
Engleski
Znanstvena područja
Prehrambena tehnologija
