Naslov
Genetic instability

Autori
Cullum, John ; Redenbach, Matthias ; Hranueli, Daslav

Vrsta, podvrsta i kategorija rada
Poglavlja u knjigama, pregledni

Knjiga
Encyclopedia of Bioprocess Technology: Fermentation, Biocatalysis, and Bioseparation

Urednik/ci
Flickinger, M.C. ; Drew, S.W.

Izdavač
John Wiley & Sons

Grad
New York (NY)

Godina
1999

Raspon stranica
1319-1326

ISBN
0-471-13822-3

Ključne riječi
mutation, selection, scale-up, strain degeneration

Sažetak
Genetic instability manifests itself as the accumulation of mutants in a population. It is a potential problem in most industrial processes as low-producing mutants are likely to outgrow the high-producing parent strain. Careful control of strain storage and inoculum preparation can limit the effects of instability. It is probable that genetic instability during scale-up is often not recognised. Detection of instability and optimisation for stability in parallel to physiological optimisation is likely to give improved yields. The type of genetic instability encountered and its detection depends on the type of organism. Both point mutations and DNA rearrangements may occur. The stability of plasmid-carrying strains depends on avoiding both segregational and structural instability. Southern blottings and PFGE can be useful for bacteria, yeast and fungi. FISH methods are important for mammalian cell cultures where changes in chromosome number and chromosome rearrangements often occur. In some cases epigenetic effects may play an important rôle in stability of cultures. Achieving and proving culture stability is an essential step in complying with GMP regulations.

Izvorni jezik
Engleski

Znanstvena područja
Prehrambena tehnologija

POVEZANOST RADA