Naslov
Regulation of HIF-1 alpha stability through S-nitrosylation

Autori
Li, F. ; Sonveaux, P. ; Rabbani, Z. N. ; Liu, S. ; Yan, B. ; Huang, Q. ; Vujasković, Željko ; Dewhirst, M. W. ; Li, C. Y.

Izvornik
Molecular Cell (1097-2765) 26 (2007), 1; 63-74

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
signaling

Sažetak
Hypoxia-inducible factor 1 (HIF-1) is a master transcriptional factor. Under normal oxygen tension, HIF-1 activity is usually suppressed due to the rapid, oxygen-dependent degradation of one of its two subunits, HIF-1alpha. Here we report that normoxic HIF-1 activity can be upregulated through NO-mediated S-nitrosylation and stabilization of HIF-1alpha. In murine tumors, exposure to ionizing radiation stimulated the generation of NO in tumor-associated macrophages. As a result, the HIF-1alpha protein is S-nitrosylated at Cys533 (through "biotin switch" assay) in the oxygen-dependent degradation domain, which prevents its destruction. Importantly, this mechanism appears to be independent of the prolylhydroxylase-based pathway that is involved in oxygen-dependent regulation of HIF-1alpha. Selective disruption of this S-nitrosylation significantly attenuated both radiation-induced and macrophage-induced activation of HIF-1alpha. This interaction between NO and HIF-1 sheds new light on their involvement in tumor response to treatment as well as mammalian inflammation process in general.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

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