Naslov
Incidence of human papillomavirus infection in population of women from eastern Croatia

Autori
Tokić, Stana ; Mihaljević, Ivan ; Pajtler, Marija ; Topolovec, Z. ; Glavaš-Obrovac, Ljubica

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
First Congress of Croatian Thyroid Society "Thyroid cancer" : abstract = Prvi kongres Hrvatskog drustva za štitnjaču " Rak štitnjače" : knjiga sažetaba ; u: Acta clinica Croatica 46 (2007) / - , 2007, 73-73

Skup
Kongres Hrvatskog drustva za štitnjaču " Rak štitnjače" (1 ; 2007)

Mjesto i datum
Split, Hrvatska, 26.10.2007. - 28.10.2007

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
human papillomavirus; women; Eastern Croatia; cervical cancer

Sažetak
Today, more then 130 types of human papillomavirus (HPV) are known, of which about 40 induces genital infections. High-risk types (HR-HPV) are frequently associated with development of cervical intraepithelial neoplasia (CIS), carcinoma in situ and cervical cancer, while low-risk types (LR-HPV) are mainly associated with genital warts. Following Papa test and HPV genotyping we have attempted to determine frequency and distribution of HR- and LR-HPV in frequently diagnosed disorders ; CIN-I, CIN-II, CIN-III, condyloma accuminata and portio suspect. DNA was isolated from cervical samples of 523 women and β -globin PCR was used for DNA quality control. PCR was inhibited in 21 (4.01%) samples. Detection and genotyping of L1 virus gene was performed by PCR-ELISA method, using consensus primers (MY09, MY11) and biotin-labeled hybridization probes specific for different HPV types. L1 negative DNA samples were additionally tested using E6/E7 consensus primers. Statistical analysis was performed by Pearson's χ 2 or Fisher exact test. HPV was determined in 228 (45.32%) specimens. Significantly higher frequency of HR-HPV genotypes was observed in CIN-II group of samples (χ 2=4.835, p=0.0286 ; OR=1.82 (95%CI 1.02-3.21)). After results were reorganized in two groups, women over and under 30 years of age, higher frequency of LR-(χ 2=5.885, p=0.015 ; OR=2.18 (95%CI 1.11-4.44)) and HR-HPV (χ 2=6.390, p=0.011 ; OR=1.88 (95%CI 1.12-3.20)) was observed in CIN-I group of younger women. Even though, our results indicate stronger association between HR-HPV and higher grades of CINs, transforming potential of LR-HPV can’ t be ignored due to their high prevalence in CIN- I group of younger women.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

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