Naslov
Allele frequencies for the fifteen short tandem repeat loci in Croatian population

Autori
Škaro, Vedrana ; Projić, Petar ; Pojskic, Naris ; Adaleta, Durmić ; Kovačević, Lejla ; Haverić, Sanin ; Primorac, Dragan ; Marjanović, Damir

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
European Journal of Human Genetics, Vol 15, Supp 1 / - : Nature publishing group, 2007, 289-289

Skup
European Human Genetics Conference

Mjesto i datum
Nica, Francuska, 16.06.2007. - 19.06.2007

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
DNA typing ; short tandem repeats ; Croatian population

Sažetak
We have analyzed the distribution of allele frequencies at fifteen autosomal short tandem repeats loci in the representative sample of Croatians. A total of 110 unrelated individuals (Caucasians) born in Croatia have been sampled for the analysis. All of them have been voluntary donors. Buccal swab have been used as the DNA source. Specimens were air-dried, placed in 1, 5 ml tubes, and immediately transported to the laboratory. The samples have been stored at -20oC until beginning of DNA analysis. The Qiagen DnaeasyTM Tissue Kit was used for DNA extraction. The AmpFlSTR® ; ; ; Identifiler® ; ; ; (ABI, Foster City, CA) has been used to simultaneously amplify by PCR 15 STR loci. The STR loci are: D3S1358, TH01, D21S11, D18S51, D2S1338, D5S818, D13S317, D7S820, D16S539, CSF1PO, D19S433, vWA, D8S1179, TPOX, and FGA. Similar amount of DNA (approx. 1 ng) was used in all PCR reactions. Total reaction volume was 12, 5 μ l. The PCR amplification has been carried out in PE Gene Amp PCR System Thermal Cycler (ABI, Foster City, CA) according to the manufacturer’ s recommendations. Electrophoresis of the amplification products was preformed on an ABI PRISM 3130 genetic analyzer Raw data have been compiled, analyzed and numerical allele designations of the profiles were obtained by using the accessory software: ABI PRISM® ; ; ; Data Collection Software v3.0 and GeneMapperΤ Μ ID Software v3.1. Deviation from Hardy-Weinberg equilibrium, observed and expected heterozygosity, power of discrimination and power of exclusion were calculated. Also, we have compared our data with data obtained from geographically neighboring European populations.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

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