Naslov
Abrupt telomere shortening and cell culture growth dynamics

Autori
Škrobot Vidaček, Nikolina ; Ćukušić, Andrea ; Ivanković, Milena ; Šatović, Eva ; Rubelj, Ivica

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
4th European workshop on Role of Telomeres and Telomerase in Cancer and Aging / - , 2008

Skup
European workshop on Role of Telomeres and Telomerase in Cancer and Aging (4 ; 2008)

Mjesto i datum
Ladenburg, Njemačka, 16.03.2008. - 18.03.2008

Vrsta sudjelovanja
Pozvano predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
telomeres; telomerase; genome stability

Sažetak
Normal human and other mammalian cell cultures of different types have a finite and predictable proliferative potential in vitro. Typically, young cultures initially have a period of rapid proliferation during which the majority of the cells divide vigorously. As the culture accumulates more divisions the growth potential gradually declines due to increase in the fraction of nondividing senescent cells. The entire culture ultimately enters the terminally nondividing state referred to as replicative senescence. Individual senescent cells appear in the culture in a sudden and stochastic fashion. It is believed that telomere shortening is a mechanism that controls these processes and numerous experimental evidence supports this idea. Telomeres are the nucleoprotein structures that cap all linear chromosomes. In normal cells, the number of telomeric repeats decrease with each cell division, due to end replication problem combined with additional -5’ exonuclease activity. Since this gradual telomere shortening can not explain sudden and stochastic onset of cell senescence, not even among sister cells arising from the same mitosis, a mechanism of abrupt telomere shortening has been proposed as a probable explanation. Since it was published, this model has been suported by numerous experiments, both structural and functional. In this work we will present experimental results that shed new light on possible molecular mechanisms of this shortening and emphesize its importance in generating heterogeneity of dividing potentials among individual cells of the culture. Using DiI FACS analysis we followed and analysed generation of heterogeneity in dividing potential of cells within growing cultures.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA