Naslov
Comparative study upon ganglioside pattern in defined regions of human cerebrum using nanoelectrospray ionization quadrupole time-of-flight mass spectrometry

Autori
Serb, Alina F. ; Flangea, Corina ; Dinca, Nicolae, Sisu, Eugen ; Harja, Florian ; Zamfir, Alina D. ; Vukelić, Željka

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, neobjavljeni rad, znanstveni

Izvornik
Research Workshop on Applications of Mass Spectrometry in Life Safety, The NATO Science for Peace and Security Programme, Herculane, Romania, Sept. 23-27, 2007 / - Arad, 2007

Skup
Research Workshop on Applications of Mass Spectrometry in Life Safety, The NATO Science for Peace and Security Programme

Mjesto i datum
Băile Herculane, Rumunjska, 23.09.2007. - 27.09.2007

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
brain region-specific ganglioside composition; comparative mass spectrometric analysis; nanoESI-QTOF MS1; tandem MS

Sažetak
Purified ganglioside mixtures isolated from frontal and occipital lobe cortex, as well as hippocampus of an adult human brain were comparatively analyzed by nano-electrospray ionization quadrupole time-of-flight mass spectrometry, under identical conditions. The brain region-specific ganglioside patterns were clearly detected by MS1 screening in the negative ion mode. Both qualitative and quantitative differences of ganglioside expression in the analyzed brain regions were observed and comparatively systematized. MS2 sequencing was employed for confirming structural identity of the detected species.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija, Temeljne medicinske znanosti

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