Naslov
Mapping and sequencing of gangliosides from anencephaly by electrospray ionization high capacity ion trap mass spectrometry

Autori
Mosoarca, Cristina ; Vukelić, Željka ; Zamfir, Alina D.

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, neobjavljeni rad, znanstveni

Izvornik
1st Advanced Research Workshop on Applications of Mass Spectrometry in Life Safety, The NATO Science for Peace and Security Programme / - Arad, 2007

Skup
1st Advanced Research Workshop on Applications of Mass Spectrometry in Life Safety, The NATO Science for Peace and Security Programme

Mjesto i datum
Băile Herculane, Rumunjska, 23.09.2007. - 27.09.2007

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
anencephalic residual brain tissue; ganglioside composition and structure; ESI-HCT mass spectrometry

Sažetak
Total ganglioside mixture was isolated and purified from a residual nervous tissue of anencephalic fetus (23 gestational weeks). Compositional and structural characterization of the purified ganglioside mixture was performed by recently developed advanced mass spectrometric approach using electrospray ionization high capacity ion trap mass spectrometer. More than 50 brain-associated ganglioside species was detected by MS1 mapping in the negative in mode. MS2 to MS4 multiple stage sequencing enabled reliable, high sensitivity structural identification of individual species directly from the complex mixture.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija, Temeljne medicinske znanosti

POVEZANOST RADA