Naslov
Mass spectrometric determination of ganglioside expression and structure in human neocortex.

Autori
Chirita, Marius I. ; Serb, Alina F. ; Sisu, Eugen ; Vukelić, Željka ; Zamfir, Alina D.

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, neobjavljeni rad, znanstveni

Izvornik
1st Advanced Research Workshop on Applications of Mass Spectrometry in Life Safety, The NATO Science for Peace and Security Programme / - Arad, 2007

Skup
1st Advanced Research Workshop on Applications of Mass Spectrometry in Life Safety, The NATO Science for Peace and Security Programme

Mjesto i datum
Băile Herculane, Rumunjska, 23.09.2007. - 27.09.2007

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
brain ganglioside composition; ganglioside structure identification; high capacity ion trap mass spectrometry; multiple stage MS

Sažetak
A protocol based on multiple stage tandem mass spectrometry (MS) for systematic and thorough investigation of the ganglioside species in human brain was developed. The unique feature of the high capacity ion trap (HCT) multiple stage MS to provide efficient dissociation, ultra-high reproducibility, sensitivity, throughput and accurate structural data, offered a reliable structural characterization of gangliosides GM1 and GD1 present in ganglioside mixture isolated from fetal neocortex ; GM1a as well as GD1a positional isomers were clearly identified. For detailed structural analysis of gangliside species, multiple stage MS enabled stepwise fragmentation at high sensitivity in up to 4 sequencing events.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija, Temeljne medicinske znanosti

POVEZANOST RADA